摘要
为了研究木霉菌株对鸭茅植株生理生化指标的影响,试验将从鸭茅根际分离筛选获得的5株优良木霉促生菌株(M1、M2、HM、LM、LS)接种到鸭茅植株上,测定丙二醛(MDA)含量,过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性及叶绿素、可溶性糖、可溶性蛋白含量。结果表明:5株木霉菌株处理的鸭茅植株叶片及根部MDA含量均低于对照,降幅分别是12.20%~31.71%,24.35%~65.38%;叶片及根部CAT活性均高于对照,增幅分别为34.56%~81.39%,8.86%~255.70%;叶片及根部POD活性较对照均有不同程度提高,增幅分别为24.40%~140.98%,16.26%~149.83%;叶片及根部APX活性较对照也有不同程度提高,增幅分别为95.86%~300.04%,47.04%~205.54%;叶片叶绿素、可溶性糖、可溶性蛋白含量增幅分别为5.98%~51.67%,3.42%~17.86%,12.98%~25.28%。说明木霉菌株能够提高鸭茅植株的抗性,从而促进鸭茅生长。
In order to study the effects of Trichoderma on plant physiological and biochemical indexes of Dactylis glomerata L. , isolated 5 excel- lent growth promoting strains of Trichoderma （ M1, M2, HM, LM, LS）from rhizosphere of Dactylis glomerata L. and inoculated into the Dactylis glomerata L. Plants. The contents of MDA, CAT activity, POD activity, APX activity and the contents of chlorophyll, soluble sugar and solu- ble protein were determined. The results showed that the contents of the five Trichoderma strains treated Dactylis glomerata L. and roots MDA were lower than the control group, the reduce ranges were 12.20%～ 31.71%, 24.35% ～ 65.38% ; the CAT activity of leaves and roots were higher than control group, rising ranges were 34.56% ～ 81.39%, 8.86% ～ 255.70% ; the POD activities of leaves and roots compared with the control group were improved, rising ranges were 24.40% ～140.98%, 16.26% ～ 149.83 % ; leaves and roots APX activities than the control group also improved to varying degrees, rising ranges were 95.86% ～ 300.04%, 24.40 ～ 140.98% ; the contents of chlorophyll, soluble sugar and soluble protein were increased of 5.98% ～ 51.67%, 3.42% ～ 17.86%, 47.04% ～ 205.54%. It was concluded that Tr/- choderma strains can improve the resistance of Dactylis glomerata L. plant, so as to promote the growth of Dactylis glomerata L.
作者
袁扬
王胤晨
曾兵
张锦华
韩玉竹
YUAN Yang WANG Yinchen ZENG Bing ZHANG Jinhua HAN Yuzhu(Institute of Animal Sciences and Veterinary Medicine, Guiyang 550000, China Rongchang Campus of Southwest University, Chongqing 402460, China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2017年第10期4-7,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
"十二五"国家科技支撑计划项目"草田系统病虫害绿色防控技术研究与示范"(2014BAD23B03-3)
贵州省科技厅重大专项"贵州山区牧草产业化生产技术研究集成与应用"(黔科合重大专项字2014年6017号)
重庆市山羊产业技术体系牧草及营养研究室建设项目
2013年重庆高校创新团队建设计划"肉牛生产技术"资助项目
2015年西南大学青年基金项目"木霉菌对牧草的促生效应研究"(20700428)
关键词
木霉菌
促生
鸭茅
抗氧化酶
抗性
Trichoderma
promoting
Dactylis glomerata L.
antioxidant enzymes
resistance
