摘要
脯氨酸积累在逆境中对植物中有保护作用,但是过量脯氨酸却表现出对植物生长发育的抑制作用。研究采用含生长素相关标签蛋白的拟南芥转基因株系,通过分析脯氨酸处理对生长素相关标签蛋白分布的影响,探讨脯氨酸对拟南芥根的生长发育产生抑制的可能原因。结果表明:脯氨酸处理影响生长素相关信号途径基因的表达;脯氨酸没有影响拟南芥根部生长素含量,而是影响生长素极性运输载体蛋白的表达;而脯氨酸含量减少的PDH过表达株系中生长素运输载体基因表达增多。研究表明,脯氨酸可能通过抑制生长素运输载体的表达,影响生长素的正常流动,从而打破生长素浓度梯度的维持,最终抑制根的生长。研究为探讨脯氨酸在植物生长发育和逆境恢复中的调节作用提供了新的依据。
Although free proline accumulation is proposed to play protective roles in many plants in response to environmental stresses, by either exogenous application or endogenous over-production, in the absence of stresses, is found to be inhibitory to plant growth. In order to explore the inner mechanism of the inhibiting effect for plant root growth, we detected the expression of auxin marker in response to proline treatment transgenic Arabidopsis auxin marker lines. The results showed that proline affected the expression of auxin genes in Arabidopsis. The content of auxin was not affected by proline, but the expression of auxin carriers was reduced. And in the overexpression lines of PDH, in which proline content was reduced, the expression of auxin carrier genes was induced. These results suggested that proline inhibited the auxin flux by inhibiting the expression of auxin carrier genes, which broke the auxin concentration gradient, resulted in inhibiting of root growth. This research will contribute to understand the role of proline in plant growth and osmotic stress.
出处
《植物生理学报》
CAS
CSCD
北大核心
2017年第8期1428-1434,共7页
Plant Physiology Journal
基金
宁夏自然科学基金(NZ16215)~~
关键词
脯氨酸
根生长
抑制
生长素运输
静止中心
proline
root growth
inhibit
auxin transport
quiescent center
