摘要
目的探讨血循环肿瘤细胞(CTCs)检测对中晚期乳腺癌疗效评价及转移监测的应用价值。方法纳入中晚期乳腺癌患者100例,于第1、2、3周期化疗前3天内分别取外周血,采用免疫磁珠阴性富集联合免疫荧光染色法进行CTCs检测;于第3周期化疗前进行影像学疗效评价,比较化疗2周期后不同转归患者的CTCs情况;所有患者于第3周期化疗开始每个月随访,直至出现新增转移灶时终止;于全部化疗结束后3个月、6个月、9个月再次分别进行CTCs检测及影像学检查。结果第1、2、3周期化疗前,中晚期乳腺癌患者的CTCs数目分别为(15.34±4.12)、(7.82±1.56)、(3.71±1.04),阳性率分别为73.00%、56.00%、29.00%,化疗1、2周期后的CTCs数目及阳性率均较初次化疗前显著降低(P<0.05);化疗2周期后,CTCs阴性患者及CTCs数目减少患者的总有效率(RR)(73.24%、66.20%)分别高于CTCs阳性患者及CTCs数目增加的患者,差异均有统计学意义(P<0.05);全部化疗结束后3个月、6个月及9个月,CTCs阴性患者的转移复发率(5.41%、10.91%、25.00%)均明显低于CTCs阳性患者。结论外周血CTCs检测可用于中晚期乳腺癌的化疗疗效评价和转移监测,对乳腺癌具有一定的预后预测价值。
Objective To investigate the application value of detection for circulating tumor cells （CTCs） in the efficacy evaluation and metastasis monitoring of patients with advanced breast cancer.Methods 100 patients with advanced breast cancer were selected in this study, and their peripheral blood were collected within 3 days before the first, second and third cycle of chemotherapy, respectively.CTCs were detected by immunomagnetic negative enrichment combined with immunofluorescence staining.The clinical efficacy was evaluated by imaging examination before the third cycle of chemotherapy, and the CTCs of patients with different outcomes after 2 cycles of chemotherapy were compared.All the patients were followed up every month at the start of the third cycle of chemotherapy till new metastasis appearance.3 months, 6 months, and 9 months after all the chemotherapy finished, the CTCs detection and imaging examination were performed once again.Results Before the first, second and third cycle of chemotherapy, the CTCs number of patients with advanced breast cancer were （15.34±4.12）, （7.82±1.56） and （3.71±1.04）, respectively.And the positive rates of those were 73.00%, 56.00% and 29.00%, respectively.Compared with the primary chemotherapy, the number and positive rates of CTCs were significantly lower when the 1 and 2 cycles chemotherapy finished （P〈0.05）.
作者
单海琳
苏瑛
周斌
邹润龙
邵清
SHAN Hai-lin SU Ying ZHOU Bin ZOU Run-long SHAO Qing(Department of Breast Surgery, Jiangyin People Hospital Affiliated to Southeast＇s University, Jiangyin 214400, China)
出处
《标记免疫分析与临床》
CAS
2017年第9期988-991,997,共5页
Labeled Immunoassays and Clinical Medicine
基金
无锡卫生局面上项目(MS201532)
关键词
中晚期乳腺癌
血循环肿瘤细胞
疗效
转移
Advanced breast cancer
Circulating tumor cells
Efficacy
Metastasis
