摘要
目的:探讨Th17细胞功能与前列腺炎病程中炎症反应、细胞凋亡的相关性。方法:收集本院收治的慢性前列腺炎患者128例,取同期在本院进行体检的健康男性50例作为正常对照组。对比两组研究对象外周血中Th17细胞比例、IL-17含量,血清中炎症因子含量,前列腺液中凋亡基因表达量的差异。采用Pearson检验评估慢性前列腺炎患者外周血Th17细胞功能与炎症反应、细胞凋亡的相关性。结果:观察组外周血中Th17细胞的比例、IL-17的含量高于正常对照组;血清中炎症因子IL-1β、IL-2、IL-8、TNF-α、M-CSF的含量高于正常对照组;前列腺液中凋亡基因BAX mRNA表达量高于正常对照组,抗凋亡基因BCL-2、livin、hPEBP4mRNA表达量低于正常对照组。经Pearson检验发现,慢性前列腺炎患者外周血中Th17细胞的比例、IL-17的含量与血清中IL-1β、IL-2、IL-8、TNF-α、M-CSF的含量以及前列腺液中BAX的mRNA表达量呈正相关,与前列腺液中BCL-2、livin、hPEBP4的mRNA表达量呈负相关。结论:慢性前列腺炎患者存在Th17细胞功能亢进,是导致患者全身炎症反应出现、前列腺细胞凋亡加剧的重要原因。
Objective To study the correlation of Th17 cell function with the inflammatory response and apoptosis in the course of prostatitis. Methods： A total of 128 patients with chronic prostatitis who were treated in our hospital were en- rolled as observation group, and 50 healthy males who ：received physical examination in our hospital during the same period were selected as normal control group. The differences in Th17 cell ratio and IL-17 levels in peripheral blood, inflammatory fac- tor levels in serum, and apoptosis gene expression in prostatic fluid were compared between the two groups. Pearson test was used to assess the correlation of Th17 cell function in peripheral blood with inflammation and apoptosis in patients with chronic prostatitis. Results.. Th17 cell ratio and IL-17 level in peripheral blood of observation group were higher than those of normal control group; inflammatory factors IL-1β, IL-2, IL-8, TNF-α and M CSF levels in serum were higher than those of normal control group; apoptosis gene BAX mRNA in prostatic fluid of observation group was higher than that of control group, while anti-apoptosis genes Bcl-2, livin and hPEBP4 mRNA expressions of observation group were lower than those of normal control group. Pearson test results showed that Thl7 cell ratio and IL-17 level in peripheral blood of patients with chronic prostatitis were positively correlated with IL-1α, IL-2, IL-8, TNF-α and M-CSF levels in serum as well as BAX mRNA expression in prostatic fluid, and negatively correlated with Bcl-2, livin and hPEBP4 mRNA expression in prostatic fluid. Conclusion： There is Th17 cell hyperfunction in patients with chronic prostatitis, and it is an important cause of the systemic inflammatory re- sponse and prostate cell apoptosis aggravation.
出处
《海南医学院学报》
CAS
2017年第15期2064-2066,2070,共4页
Journal of Hainan Medical University
基金
东莞市科技局项目(201650715001064)
