摘要
目的通过体内外实验研究探讨BH3-only模拟物S1抑制小鼠黑色素瘤B16细胞生长的机制。方法体外实验分为对照组、S1组(5μmol/L),MTT法检测细胞存活率;Hoechst 33342染色法检测细胞核形态变化;生化试剂盒检测细胞葡萄糖消耗量及乳酸生成量。体内实验将B16细胞种植于BALB/C小鼠腋下,14天后腹腔注射S1(0.6mg/kg),隔天给药,共计28天。实验分为对照组、S1组。免疫组化法检测移植瘤糖代谢相关蛋白HK2、PKM2的表达变化。结果与对照组相比,S1组B16细胞生存率明显降低,差异有统计学意义(P<0.05);凋亡核明显增多;葡萄糖摄取减少,乳酸分泌减少。与对照组相比,S1组移植瘤中HK2、PKM2蛋白表达明显降低,差异有统计学意义(P<0.05)。结论S1在体内和体外均可抑制小鼠黑色素瘤B16细胞生长,可能是通过抑制葡萄糖代谢诱导细胞凋亡发挥抑瘤作用。
Objective To investigate the mechanism of BH3-only mimetic S1 in the inhibition of melanoma B16 cells growth. Methods In vitro,the experiment was divided into control group and S1 group （5 μmol / L） ,MTT assay was used to detect cell viability. Hoechst 33342 staining method was used to detect the morphology changes of the nucleus. The biochemical kit was used to detect glucose consumption and lactic acid production. In vivo experiments, B16 cells were implanted in BALB/C mice,The mice were treated with S1 （0.6 mg / kg） for 14 days and killed after 28 days. The expression of HK2 and PKM2 were detected by immunohistochemistry. Results Compared with the control group ,the cell viability of B16 cells in S1 group was significantly lower than the control group （P〈0.05）. The apoptotic nuclei of B16 cells in S1 group were significantly increased; the glucose uptake and lactate secretion of B16 cells in S1 group decreased. Compared with the control group,the expression of HK2 and PKM2 protein in $1 group was significantly lower （P〈0.05）. Conclusion S1 can inhibit the growth of melanoma B16 cells in vivo and in vitro,and may be related to the inhibition of glucose metabolism.
作者
李亚平
吴瑶
张娟娟
张丽超
苏静
LI Ya-ping WU Yao ZHANG Juan-juan et al(People＇s Hospital of Jilin Province ,Changchun 130021 ,China)
出处
《中国实验诊断学》
2017年第9期1601-1604,共4页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金面上项目(81472419
81272876)
