摘要
为了提高硫酸软骨素的品质,采用Sevag试剂对硫酸软骨素提取液进行脱蛋白处理,通过单因素试验和正交试验对脱蛋白工艺进行优化,得到最佳条件为:氯仿与异戊醇体积比2:1,Sevag试剂用量为硫酸软骨素提取液体积的1/2,处理时间25min,处理次数3。各因素对脱蛋白效果影响大小依次为氯仿异戊醇比例>处理时间>处理次数>Sevag试剂用量。在该最佳条件下,硫酸软骨素保留率为87.98%,脱蛋白率52.15%。经190~350nm紫外扫描,未见280nm处蛋白质特征吸收峰;经红外光谱分析,硫酸软骨的构型未发生变化,说明该方法稳定可靠,适合于工业化硫酸软骨素的脱蛋白。
In order to promote the quality of chondroitin sulfate,Sevag reagent was used to deprotein the extraction of chondroitin sulfate through the single factor and the orthogonal tests to optimize the process of deproteinization.The optimal conditions were ratio of chloroform to isoamyl alcohol 2：1（V/V）,Sevag reagent dosage1/2fold compared with the volume of chondroitin sulfate extraction,treated for 25 min and repeated treatment for 3times.The factors influenced the extraction in the order of ratio of chloroform to isoamyl alchohol,treatment time,treatment times,and Sevag reagent dosage.Under the optimal conditions,the retention rate of chondroitin sulfate was 87.98%,and the deproteinization rate was52.15%.No specific absorption of 280 nm was observed with ultraviolet（UV）scanning from 190 to 350nm,and the configuration of chondroitin sulfate was confirmed untransformed with Fourier-transform infrared spectroscopy（FTIR）analysis.The results suggested that this deproteinization method was stable,reliable and suitable for deproteinization of chondroitin sulfate in industry.
出处
《食品与机械》
CSCD
北大核心
2017年第7期184-188,共5页
Food and Machinery
基金
湖南省科技计划重点研发与技术创新项目(编号:2016NK3093)
关键词
Sevag试剂
硫酸软骨素
脱蛋白
红外光谱
Sevag reagent
orthogonal test
chondroitin sulfate
deproteinization
Fourier-transform infrared spectroscopy（FTIR）
