摘要
目的探讨高级别宫颈鳞状上皮内瘤变(HSIL)锥切手术前后阴道菌群构成的差异。方法选择2015年12月至2016年8月南方医科大学珠江医院、广东省人民医院收治的HSIL患者20例,分别采集其锥切手术前与手术后3个月的阴道分泌物,同时检测高危型HPV-DNA含量(HC2法);随后对每个样本进行细菌总基因组DNA提取、16S rRNA V4区基因扩增及采用Illumina高通量测序技术对扩增的PCR产物进行测序等步骤,然后通过BIPES生物信息分析方法比较HSIL患者手术前后阴道菌群物种丰度和结构的差异。结果 HSIL患者术前高危型HPV-DNA均为阳性,术后3个月第1次复查均转阴性。HSIL患者手术前的阴道菌群以惰性乳酸杆菌、加德纳菌属、普氏菌属、奇异菌属等为优势菌属,而卷曲乳酸杆菌含量低。在锥切手术后,惰性乳酸杆菌、加德纳菌属、普氏菌属、奇异菌属等则明显降低,卷曲乳酸杆菌则有升高趋势。术后则以卷曲乳酸杆菌、脲原体、普氏菌属、肠球菌属等为优势菌属。结论高级别宫颈鳞状上皮内瘤变患者经手术去除病变后,高危型HPV含量显著减少甚至消失,其阴道菌群出现了向常见阴道优势菌属转变的趋势。提示HPV可能与阴道菌群构成密切相关且相互影响。
Objective To explore the diversity of the vaginal flora before and after cervical conization in patients with high-grade squamous intraepithelial lesion（HSIL）. Methods Swabs were collected from December 2015 to August 2016 in Guangdong General Hospital and Zhujiang Hospital from 20 cases of HSIL patients before cervical conization and three months after cervical conization. Microbiota composition,alpha diversity and beta diversity among two groups were determined by analyzing the 16 S rRNA V4 hypervariable region amplified from total genomic DNA prepared from the swabs.Results L.iners,Gardnerella,Prevotella and Atopobium was the predominant species in HSIL patients before cervical conization,and the content of L.crispatus decreased significantly.While after cervical conization,content of L.iners,Gardnerella,Prevotella and Atopobium decreased,while content of L.crispatus increased.Conclusion High abundant of L.iners,Gardnerella,Prevotella and Atopobium and low abundant of L.crispatus maybe was the risk factors of HR-HPV infection.
出处
《中国实用妇科与产科杂志》
CAS
CSCD
北大核心
2017年第9期949-954,共6页
Chinese Journal of Practical Gynecology and Obstetrics
