摘要
本研究旨在探明喜树碱植物水培体系中化感物质的种类及其长期积累对植物生产的作用。建立了喜树幼苗和野生型蛇根草水培体系根系分泌物的新分离富集方法:首先以硅胶填充固相萃取柱萃取水培液根系分泌物,然后取出填充硅胶加到硅胶层析柱上层进行柱层析分离,再经洗脱后,用GC-MS技术分析不同组分的洗脱液。从喜树水培根系分泌物中检测到1,1,2,2-四氯乙烷、N,N-二甲基苯胺、二苯铬等11种化感物质;在广州蛇根草水培根系分泌物中检测到邻苯二甲酸二丁酯、苯硒酚、二苯铬等14种化感物质。2种含喜树碱植物水培根系分泌物中化感物质的大量分离富集和分析,为清除水培体系的生长抑制物、优化水培体系、实现喜树碱的高效生物合成奠定基础。
The purpose of this study is to identify the types of allelopathic compounds in a hydroponic systemand clarify the effects of their long time accumulation in hydroponic root system on plants. A new technology ofseparating and enriching root secretions from acuminata seedlings and wild Ophiorrhiza cantoniensiscultivated was established： the solid phase extraction column filled with silica gel was used to extract rootsecretions from the hydroponic solution; then the silica gel was taken out and added in the top of the slicacolumn chromatography for column chromatography isolation. After elution, the GC-MS was used to analyzethe eluant with different constituents. 11 allelopathic compounds were found in root secretions of Camptotheca acuminata Decne such as 1,1,2,2-tetrachloroethane, N,N-Dimethylaniline, dibenzene-chromium and so on. 14 allelopathic compounds were identified in root secretions of Ophiorrhiza cantoniensis Hace such as Dibutylphthalate, Dioctyl phthalate, dibenzene-chromium and so on. The isolation, enrichment and analysis ofallelochemicals in root secretions of two plant species containing camptothecin laid the foundation foreliminating growth inhibitors of hydroponic system and optimizing hydroponic system.
出处
《中国农学通报》
2017年第23期10-15,共6页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"在线富集生物反应器培育特异性花生毛状根合成白藜芦醇"(201376281)
"新型苦参碱衍生物的分子优化合成
杀虫活性构效关系及机理研究"(21406274)
广东省大学生创新训练项目"花生壳白藜芦醇提取工艺优化及种间含量分析"(201411347034)
"几种天然产物对黑色素瘤细胞增殖及其黑色素合成机制的影响"(201511347056)
关键词
喜树
广州蛇根草
喜树碱
水培体系
化感化合物
Camptotheca acuminata Decne
Ophiorrhiza cantoniensis Hace
camptothecin
hydroponic system
allelopathic compounds
