摘要
以金叶紫穗槐(Amorpha fruticosa‘Jinye’)为外植体,探讨了不同生长调节对外植体分化、增殖和生根的影响。结果表明,(1)金叶紫穗槐组织培养最适宜的外植体是半木质化带芽茎段,以70%乙醇30 s+0.1%升汞(HgCl_2)消毒4~5 min为最好,外植体发芽率可达66.7%~70.0%。(2)适宜的增殖培养基为MS+6-BA 0.2 mg/L+NAA 0.01 mg/L,适宜的生根培养基为1/2MS+IBA 0.5 mg/L。(3)生根试管苗适宜的开瓶炼苗时间为2~3 d,在一年中的3~5月,选择基径≥0.3 mm的试管苗移栽到蛭石培养基上能够获得93.3%的成活率。
Using Amorphafruticosa ＇Jinye＇ as explants, influences of different plant growth hormones on differentiation,multiplication and rooting were examined. The results showed that（1）the optimal explant material was tender stem with single bud, sterilized 30 s in 70% ethanol and 4-5 rain in 0.1% HgCl2,the germination rate of explants was 66.7%-70.0%. （2）The optimal subculture was MS＋6-BA 0.2 mg/L＋NAA 0.01 mg/L,and rooting media was 1/2MS＋IBA 0.5 mg/L.（3）the glass seedling rooting condign open the glass bottle hardened times was 2-3 d,than the diameter ≥0.3 mm glass seedling was transplanted on vermiculite,the survive rates of glass seedling were 93.3% in march and may of a year.
出处
《湖北农业科学》
2017年第16期3148-3151,共4页
Hubei Agricultural Sciences
基金
2015年北京市教育委员会科技计划一般项目(KM201512448002)
