摘要
目的探讨卵巢癌患者外周血中CD4+T细胞亚群:辅助T细胞(Th,包括Th1、Th2、Th17)及调节性T淋巴细胞(Treg)表达、平衡关系的变化,以及在卵巢癌发病机制中的作用。方法流式细胞术检测30例卵巢癌疾病组及20例健康对照组外周血中Thl、Th2、Thl7、Treg表达占CD4+T细胞的百分率,并计算Th1/Th2、Th17/Treg的比值。结果卵巢癌患者外周血中Th2、Th17及Treg亚群占CD4+T细胞的百分率明显高于健康对照组(P<0.05),而Th1百分率低于健康对照组(P<0.05),各亚群变化与临床分期相关,但与组织类型及细胞分化无关(P>0.05)。卵巢癌组Th1/Th2比值降低,与对照组比较有统计学意义(P<0.05),卵巢癌患者存在Th2方向极化;卵巢癌组Treg/Th17比值升高,与对照组比较有统计学意义(P<0.05)。结论卵巢癌患者外周血中存在CD4+细胞亚群表达异常,存在Th1/Th2、Treg/Th17比值失衡,为卵巢癌临床免疫治疗提供理论支持。
Objective To investigate the changes in the percentages and balance of CD4＋T cell subsets including T helper cells （Thl, Th2, and Thl7） and T regulatory cells （Treg） in patients with ovarian cancer. Methods Peripheral blood samples were collected from 30 patients with ovarian cancer and 20 healthy subjects for analysis of the percentages of Thl, Th2, Thl7 and Treg using flow cytometry. Results Compared with the control subjects, the patients with ovarian cancer showed significantly increased percentages of Th2, Thl7 and Treg （P〈0.05） but significantly decreased percentage of Th1 in the peripheral blood of patients with ovarian cancer （P〈0.05）. The changes in CD4＋ T cell subsets were significantly correlated with the clinical stage of the tumor （P〈0.05） but not with the histological type or cell differentiation （P〉0.05）. The Th1/Th2 ratio was significantly decreased in ovarian cancer patients （P〈0.05） with obvious Th2 polarization compared with control group. The Treg/Th17 ratio was significantly increased in ovarian cancer patients （P〈0.05）. Conclusion Patients with in ovarian cancer have abnormal expressions of CD4＋T cell subsets in the peripheral blood with Th1/Th2 and Treg/Th17 imbalance, and these findings provide evidence for clinical immunotherapy of ovarian cancer.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2017年第8期1066-1070,共5页
Journal of Southern Medical University
基金
国家自然科学基金(81603155)
安徽省教育厅一般项目(KJ2015B047by)
蚌埠医学院自然科学基金重点项目(BYKY1606ZD)~~
