摘要
建立超高效液相色谱串联三重四级杆质谱法(UPLC-MS/MS)同时测定西洋参中8种人参皂苷类成分(人参皂苷Rb1、人参皂苷Rb2、人参皂苷Rb3、人参皂苷Re、人参皂苷Rc、人参皂苷Rd、人参皂苷Rg1和拟人参皂苷F11)的定量分析方法。采用Waters Acquity BEH C18柱(100 mm×2.1 mm,1.7μm)色谱柱,流动相为0.1%甲酸水(A)-乙腈(B),梯度洗脱,流速0.25 m L/min,柱温35℃。电喷雾电离源(ESI),采用多反应检测模式(MRM),以保留时间及定性离子对之间的相对丰度定性,以定量离子对峰面积进行定量。定量分析西洋参中8种人参皂苷类成分在考察的浓度范围内呈良好的线性关系(r>0.99);回收率和RSD分别在95.65%~103.34%,0.38%~4.33%。本研究所建立的同时测定西洋参中8种皂苷类成分的UPLC-MS/MS定量分析方法简便、快捷、准确,可为综合评价西洋参的质量提供参考。
To establish an ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） method for the simultaneous determination of 8 major compounds （ Ginsenoside Rb3, GinsenosideRc, Ginsenoside Rb2 , Ginsen- oside Rb2, Ginsenoside Rd, Ginsenoside Re, Ginsenoside Rg2, Pseudoginsenoside Fn ） in Panax quinquefolium. Using Waters BEH C18 column （2. 1 mm x 100 mm,l. 7 jjim） with a gradient solvent system of acetonitrile-0. 1% formic acid in water as mobile phases, flow rate： 0. 25 mL/ m i n , column temperature ： 35 . Multiple reactions monitoring （ M R M ）mode was used as detection mode with electro-spray ionization （ESI） source. The retention time and detected relative a-bundance of ion pairs were used for qualitative determination, the detected peak areas of ion pairs were used for quantita-tive determination. Under the optimized chromatographic conditions, good separation for 8 targeted compounds were ob-tained. Satisfactory linearity was achieved with wide linear range and fine determination coefficient （r 〉0.999） ,the o-ver-all recoveries were ranged from 95.65% -103. 34% with the RSD ranging from 0. 38% -4. 33% . It is the report a-bout simultaneous analysis of 8 major ginsenosides components in P. quinquefolium by using U P L C - M S / M S method, which affords highly sensitive, specific, speedy and efficient method for quality control of P. quinquefolium.
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2017年第9期1529-1534,共6页
Natural Product Research and Development
基金
国家自然科学基金(81373548)
国家自然科学基金(81673871)
2016哈尔滨商业大学研究团队支持项目(2016TD009)
