摘要
为明确感染柑橘衰退病毒(Citrus tristeza virus,CTV)的柑橘对不同翅型橘蚜取食行为的影响,通过刺探电位图谱技术(electrical penetration graph,EPG)对褐色橘蚜和棉蚜在感染CTV植株上的取食行为进行持续记录,与健康植株进行比较分析。结果表明,褐色橘蚜无翅型在感病植株的参数均与有翅型无显著差异;棉蚜无翅型在感病植株上的非刺探波(no penetration,np波)和路径波(C波)的总持续时间、电势落差(potential drops,pd波)的次数均显著小于有翅型,C波和刺探的次数均极显著小于棉蚜有翅型。褐色橘蚜无翅型在感病植株上的pd波的次数和韧皮部分泌唾液波(E1波)的平均持续时间显著小于棉蚜无翅型;褐色橘蚜有翅型在感病植株上的C波的次数和总持续时间、刺探的次数、pd波的次数和总持续时间均极显著小于棉蚜有翅型,韧皮部被动吸食波(E2波)的总持续时间和平均持续时间均极显著大于棉蚜有翅型。褐色橘蚜无翅型在感病植株上的刺探和pd波的次数均显著高于健康植株,但总刺探时间相反,np波、C波和非韧皮部阶段的总持续时间极显著高于健康植株,E2波的总持续时间极显著短于健康植株。表明柑橘感病状态对无翅型和有翅型的影响是相似的;柑橘感染CTV对褐色橘蚜无翅型的取食行为影响明显,且偏好健康植株,从而促进病毒的传播。
To evaluate the effects of Citrus tristeza virus-infected citrus plants on the feeding behavior of different wing dimorphisms, feeding behaviors between alate and apterous of Toxoptera citricida （Kirkaldy）and Aphis gossypii Glover on the CTV-infected plants were explored using the electrical penetration graph （EPG） techniques and comparing to healthy citrus plants. The results showed that apterous of T. citricida on CTV-in- fected citrus plants had no significant difference comparing to alate one＇ s. Some EPG parameters （ total time of np and C ,number of potential drops） of apterous of A. gossypii Glover on the CTV-infected was significantly smaller than those of alate of A. gossypii Glover, while number of probes and C on apterous of A. gossypii Glover were extremely significantly smaller than those of alate of A. gossypii Glover. The number of pd and duration time of E 1 of apterous of T. citricida （Kirkaldy） on the CTV-infected were significantly smaller than those of apterous of A. gossypii Glover. However, EPG parameters of alate of T. citricida （Kirkaldy） on num- ber and total duration time of C, number of probes, number and total duration time of pd were significantly smaller than those of alate of A. gossypii Glover, but bigger on total duration time and average duration time of E2. For apterous of T. citricida（Kirkaldy）on the CTV-infected plants, number of probes and number of pd were significantly longer than healthy citrus plants, but total duration of probing was opposite. The duration time of np and C waveforms, total duration of no phloematic phase of apterous T. citricida on CTV-infected citrus plants were longer than healthy citrus plants; the duration time of E2 and percent of E2 were shorter than healthy citrus plants. These results implied that some similarity of EPG parameters of alate and apterous of T. citricida （Kirkaldy） and Aphis gossypii Glover feeding were observed on the infected plants. The effect of CTV-infected citrus plants on the feeding behavior of apterous T. citricida was obvious, and apterous T. citricida prefered healthy citrus plants.
出处
《山地农业生物学报》
2017年第4期13-20,共8页
Journal of Mountain Agriculture and Biology
基金
国家公益性行业(农业)科研专项(201203076)
关键词
刺吸电位图谱
柑橘衰退病
有翅蚜
无翅蚜
病毒传播
electrical penetration graph
citrus tristeza virus
alate aphid
apterous aphid
virus transmission
