摘要
背景:胃癌的发病率和死亡率在全球居高不下,因此亟需探索胃癌诊断和治疗的新靶点。核蛋白1(Nupr1)具有多种生物学功能,尤其是在恶性肿瘤发生、进展过程中的作用突出。目的:探讨Nupr1在胃癌中的表达情况及其临床意义。方法:利用实时荧光定量PCR检测72例胃癌组织及其配对癌旁组织中的Nupr1 mRNA表达,以蛋白质印迹法和免疫组化技术检测Nupr1蛋白表达及其细胞定位,分析Nupr1表达与胃癌临床病理特征的关系。结果:胃癌组织中Nupr1 mRNA和蛋白表达均显著高于癌旁组织(P<0.05),两者表达变化趋势相一致。Nupr1蛋白主要表达于细胞质,细胞核内也可见少量表达。胃癌组织中的Nupr1 mRNA表达与肿瘤体积、分化程度、病理类型、Bormann分型、浸润深度、淋巴结转移、远处转移和TNM分期显著相关(P<0.05),与患者性别、年龄、肿瘤部位无明显相关性(P>0.05)。结论:Nupr1在胃癌组织中高表达并可能与肿瘤侵袭、转移、进展相关,可作为胃癌早期诊断和预后评估的新标记物,并可能成为胃癌治疗的潜在靶点。
Background：The morbidity and mortality of gastric cancer remain high worldwide,and it is urgent to explore new targets for the diagnosis and treatment of gastric cancer.Nuclear protein 1（Nupr1）has a variety of biological functions,especially in the tumorigenesis and development of the malignancies.Aims：To investigate the expression and clinical significance of Nupr1 in gastric cancer.Methods：Quantitative RT-PCR was used to determine the mRNA expression of Nupr1 in 72 cases of gastric cancerous tissue and the paired paracancerous tissue.Western blotting and immunohistochemistry were employed to detect the protein expression and cellular localization of Nupr1.The correlation of Nupr1 with the clinicopathological characteristics of gastric cancer was analyzed.Results：Expressions of Nupr1 mRNA and protein in gastric cancer were both significantly higher than those in paracancerous tissue（P〈0.05）,and their expressions were coincident.Nupr1 protein was expressed mainly in cytoplasm,and the nuclear expression was reduced.Expression of Nupr1 mRNA in gastric cancer was significantly correlated with tumor size,differentiation,pathological type,Bormann’s classification,depth of invasion,lymph node and distant metastases and TNM staging（P〈0.05）,and was not correlated with gender,age and site of tumor（P〈0.05）.Conclusions：Nupr1 is overexpressed and correlated with invasion,metastasis and progression of gastric cancer.It can be used as a novel biomarker for early diagnosis and prognosis evaluation,and as a potential target for treatment of gastric cancer.
出处
《胃肠病学》
2017年第8期460-464,共5页
Chinese Journal of Gastroenterology
关键词
胃肿瘤
疾病恶化
核蛋白类
实时聚合酶链反应
印迹法
蛋白质
免疫组织化学
Stomach Neoplasms
Disease Progression
Nucleoproteins
Real-Time Polymerase Chain Reaction
Blotting
Western
Immunohistochemistry
