摘要
目的:建立可用于重楼药材中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的一测多评定量分析方法。方法:分别以重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ作为内参物,计算4种重楼皂苷间的相对校正因子,进而对重楼植物中的重楼皂苷进行定量分析,并将此计算结果与外标实测值进行比较,以评价"一测多评法"的准确性和可行性。结果:以重楼皂苷Ⅰ作为内参物计算其余3种皂苷含量时,所得到一测多评法的计算结果与外标法无显著差异。结论:所建立的"一测多评法"测定重楼属植物中的4种重楼皂苷准确、快速且耐用性良好,为采用不同的评价方法对重楼植物品质评价提供了科学依据。
OBJECTIVE This study aimed to simultaneously determine four polyphyllins（polyphyllin Ⅰ,polyphylliⅡ,polyphyllin Ⅵ,polyphyllin Ⅶ）by quantitative analysis of multi-components with a single-marker（QAMS）in different paris.METHODS Respectively using the polyphyllin Ⅰ,the polyphylliⅡ,the polyphyllin Ⅵ,the polyphyllin Ⅶ as an internal marker to establish the relative correction factor（RCF）between four polyphyllins,and the RCF would be used to calculated their contents,comparing with the result between external standard method and QAMS,In order to appraise this method is accuracy and feasible.RESULTS With the polyphyllinⅠas the internal standard substance,No signiticant difference were found in the quantitative results of four polyphyllins by external standard method and QAMS method.CONCLUSION The established QAMS method which used to determinate the four polyphyllins in parisis accuracy,rapid and stable,It provides a scientific reference for using different method to appraise the quality of paris.
出处
《中国医院药学杂志》
CAS
北大核心
2017年第16期1612-1616,1621,共6页
Chinese Journal of Hospital Pharmacy
基金
云南省科技厅面上项目(编号:2013FB059)
云南省大学生创新创业计划项目(编号:201610679009
201610679003)
