摘要
选择7种不同类别的共代谢基质,以功能菌群DDMZ1为初始菌群,以偶氮染料活性黑5为目标污染物,采用静置培养方式(兼氧培养)进行一系列静态批次实验,研究脱色功能菌群DDMZ1在不同共代谢基质(蔗糖、葡萄糖、果糖、葡萄糖+果糖、牛肉膏、蛋白胨、碳酸钠)作用下脱色性能及菌群群落结构的变化情况。脱色结果表明,添加果糖共基质能促进菌群脱色性能,对浓度为500 mg·L^(-1)的活性黑5作用72 h后,脱色率达到88.96%。高通量测序分析结果表明,活性黑5脱色菌群群落结构因添加的共代谢基质不同而明显不同。在微生物分类属水平上,Acinetobacter菌属在经蛋白胨、牛肉膏和碳酸钠作用后的样品中占据主导地位,而Lactococcus菌属和Burkholderia菌属则在经蔗糖、葡萄糖、果糖、葡萄糖+果糖作用后的样品中含量偏高。其中,乳球菌属Lactococcus在以果糖为共代谢基质样品中达到其最大比例,结合脱色结果推测乳球菌属Lactococcus是重要的优势功能菌属。综上可知,相对于初始菌群DDMZ1,有机氮源和无机碳源共基质对菌群的群落结构影响较小,糖类共基质对菌群的群落结构影响较大,其中果糖能更好地富集乳球菌属Lactococcus类脱色功能菌群,从而优化脱色功能菌群群落结构比例,促进菌群脱色效果。
In this paper, seven types of substrates were used as co-metabolic substrates, the functional bacterial flora DDMZ1 was applied as the initial bacterial flora, and an azo dye, reactive black 5, was chosen as the target pollutant. The effects of different cometabolic substrates （sucrose, glucose, fructose, glucose ＋ fructose, beef extract, peptone, sodium carbonate） on the decolorization performance and community structure of bacterial flora DDMZ1 on decolorization of reactive black 5 were studied by the stationary culture method. Decolorization results showed that added fructose co-substrate promoted bacterial decolorization performance. Under 500 mg·L^-1 of reactive black 5, culturing for 72 h, the decolorization rate reached 88.96%. Highthroughput sequencing analysis results showed that the bacterial community structure significantly differed with different cometabolic substrates. On the classification of genus level, Acinetobacter spp. occupied the leading position in samples with peptone, beef extract and carbonate as co-substrates, whereas contents of Lactococcus spp. and Burkholderia spp. were higher in samples with sucrose, glucose, fructose, glucose ＋ fructose as co-metabolic substrates. Among them, Lactococcus reached its maximum ratio in sample with fructose as co-metabolic substrate, which indicated that Lactococcus might function as an important dominant genus in decolorizing process of reactive black 5. To sum up, compared to the initial flora DDMZ1, organic nitrogen sources and inorganic carbon co-metabolic substrates had little effects on the community structure, while sugars as co-metabolic substrates significantly affected the community structure. Furthermore, fructose was able to enrich Lactococcus as functional decolorizing bacteria, and optimized the bacterial community structure, thus promoting bacterial decolorization effects.
出处
《生态学杂志》
CAS
CSCD
北大核心
2017年第9期2572-2580,共9页
Chinese Journal of Ecology
基金
国家自然科学基金项目(51508083
21377023)
中央高校基本科研业务费专项资金(2232015D3-22)
国家重点研发计划项目(2016YFC0400501)资助
关键词
印染废水
脱色菌群
共代谢基质
活性黑5
高通量测序
dyeing wastewater
decolorizing bacteria
co-metabolic substrate
reactive black 5
high-throughput sequencing.
