摘要
以粤糖新品种03-373的成熟甘蔗种茎作为研究材料,研究不同植物生长调节剂配比对愈伤组织脱分化和再分化诱导、茎尖与腋芽萌发诱导、丛芽增殖培养、生根培养的影响。结果表明,适合粤糖03-373的最佳综合组培方案为:诱导愈伤组织脱分化适宜培养基为MS+2,4-D1.0mg/L,诱导愈伤组织再分化的适宜培养基为MS+BA0.5mg/L+IBA0.01mg/L,诱导茎尖与腋芽萌发的适宜培养基为MS+BA1.0mg/L+IBA0.04mg/L,丛芽继代增殖适宜培养基为MS+BA1.0mg/L+IBA0.04mg/L,生根培养基以1/2MS+ABT0.20g/L+IBA0.05mg/L+活性碳0.5g/L;继代培养基中添加不同浓度椰汁、水解酪蛋白和酵母提取物,组培苗黄化率由低到高依次为椰汁<水解酪蛋白<酵母提取物<对照;采用简易的沙培方式,组培苗的假植成活率达99%以上,大田移栽成活率达98%。
Experiments were carried out using stem of a new sugarcane variety Yuetang 03-373 as materials, the best synthetic way of tissure culture was obtained by different exogenous hormones ratio for inducing dedifferentiation and redifferentiation of callus, inducing germination of shoot-tip and axillary bud, clustered buds cultivation, multiplication during subculture and rooting. The results showed that for Yuetang 03-373, MS ＋ 2, 4-D mg/L was the optimal medium for callus induction, MS＋BAO.5 mg/L ＋ IBAO.O1 mg/L was the optimal medium for buds differentiation from callus induction, MS＋ BA1.0 mg/L ＋ IBA 0.04 mg/L was the optimal medium for germination of shoot-tip and axillary bud, MS ＋ BA1.0 mg/L ＋IBA0.04 mg/L was the optimal proliferation medium, and the best medium for rooting was 1/2MS ＋ ABTO.20 g/L ＋ IBA0.05 mg/L ＋ Ac0.5 g/L. Etiolation rates of plants were in order from low to high among coconut juice, casein hydrolysate, yeast extract and control in the subculture medium. The survival percentage of transplant was up to 99% with the simpal sand medium, transplanting survival rate was up to 98% in the soil.
出处
《广东农业科学》
CAS
2017年第5期13-18,F0002,共7页
Guangdong Agricultural Sciences
基金
广东省科技计划-科技基础条件建设项目(2015A030302009)
湛江市科技计划项目(2015A03014)
关键词
甘蔗
粤糖03—373
组培快繁
愈伤组织培养
茎尖和腋芽培养
sugarcane
Yuetang 03-373
tissue culture and rapid propagation
callus culture
shoot-tip and axillary bud culture
