摘要
目的评价不同参数组合电针对大鼠神经病理痛的镇痛效应,筛选出电针治疗神经病理性疼痛的最佳参数组合。方法 30只SD雄性大鼠,随机分为空白对照组、模型组、2 Hz组、120 Hz组和2/120 Hz组,每组各6只。采用脊神经结扎(SNL)的方法建立大鼠神经病理痛模型。用电针干预大鼠患侧"足三里"和"昆仑"穴。于造模前,造模后24 h,治疗第1、3、5、7、9、10天检测大鼠右后足机械缩足阈值(PWTs)。用放射免疫分析法(RIA)法检测下丘脑脑啡肽(ENK)和β-内啡肽(β-END)的含量。结果各时间点,2 Hz电针组大鼠痛阈高于模型组(P<0.05或P<0.01);电针干预后第3、5、7天,2 Hz组大鼠痛阈高于120 Hz组和2/120 Hz组(P<0.05或P<0.01);电针干预后第1、7、9、10天,2/120Hz组和120Hz组大鼠痛阈高于模型组(P<0.05或P<0.01)。各电针组大鼠下丘脑β-END含量均高于空白对照组和模型组(P<0.01);2/120 Hz大鼠下丘脑ENK含量均高于空白对照组、模型组、2 Hz组和120 Hz组(P<0.05或P<0.01)。结论不同参数组合电针均能减轻大鼠神经病理性疼痛,但不同参数组合电针镇痛效果有所不同,电针治疗神经病理性疼痛与内啡肽分泌有关,神经病理痛早期以2 Hz/50μs电针为宜,后期不同参数组合电针镇痛效果相当。
Objective To evaluate the analgesic effect of neuropathic painmodel rat of electroacupuncture with different parameter combinations, select the best parameter combination of electroacupuncture treatment forneuropathic pain. Meth- ods 30 SD male rats were randomly divided into normal group （N group）, model group （M group）, 2 Hz group, 120 Hz group, and 2/120 Hz group, with 6 rats in each group. Neuropathic pain model was established by spinal nerve ligation （SNL）. EA groups were given electroacupuncture on ipsilateral of Zusanli （ST36） and Kunlun （BL60）. The Paw withdraw thresholds of each rat were measured as an assessment of nociception before and 24 hours after operation and at the 1st, 3rd, 5th, 7th, 9th, lOth day after the treatment. The ENK and 13-END concentration of hypothalamus were mea- sured by RIA. Results PWTs of 2 Hz groups was higher than M group in every time point （P 〈 0.05 or P 〈 0.01）; at the 3rd, 5th, 7th after EA, the PWTs of 2 Hz group were higher than 120 Hz group and 2/120 Hz group （P 〈 0.05 or P 〈 0.01）; at the 1st, 7th, 9th, 10th after EA, the PWTs of 120 Hz group and 2/100 Hz group were higher than M group （P 〈0.05 or P 〈 0.01）. The β-END concentration of hy- pothalamus of EA groups was higher than M group and N group （P 〈 0.01）. The ENK concentration of hypothalamus of 2/120 Hz EA group was higher than M group, N group, 2 Hz EA group and 120 Hz EA group （P 〈 0.05 or P 〈 0.01）. Conclusion Different parameter com- binations eleetroacupuncture have analgesic action for neuropathic pain modal rat, but the analgesia effect is different. Electroacupunetrue is effective for neuropathic painrelated to the secretion of endorphins. At early stage of neuropathic pain,2 Hz/50 p,s electroacupuncture is advisable, while at late stage of neuropathic pain, different parameter combina- tions electroacupuncture is similar efficacious.
出处
《中国医药导报》
CAS
2017年第23期18-21,共4页
China Medical Herald
基金
浙江省中医药科研基金项目(2015ZA116)
浙江省重点科技创新团队建设项目(2013TD15)
关键词
电针
频率
神经病理痛
镇痛
参数
Electroacupuncture
Frequencies
Neuropathic pain
Analgesia
Parameters
