摘要
目的进行人牙周膜细胞(PDLCs)三维立体培养的体外实验研究,初步探讨两种支架材料应用于牙周组织工程的可行性,为进一步研究牙周组织生理病理及牙周组织工程奠定实验基础。方法组织块法培养人PDLCs,传代扩增后,接种于脱矿松质骨基质(CBM)和纳米羟基磷灰石材料(nHAC)两种三维支架上,体外继续培养3d,进行细胞计数和扫描电镜观察。结果人PDLCs在两种支架材料上均能形成良好贴附并增殖,扫描电镜可见两种支架材料均具有良好的多孔网状结构,细胞在支架材料上生长旺盛,伸展充分。结论CBM和nHAC均有望成为牙周组织工程的支架材料。
Objective Three-dimensional cultured model o f human periodontal ligament cells was established to evaluate the feasibility of the two materials as the scaffolds of periodontal tissue engineering.Methods Human periodontal ligament cells cu ltured in vitro were col-lected and seede d on three-dimensio nal framework of cancellous bone matrix(CBM)and nano-HAp /collagen(nHAC)composite.The cell growth in the scaffolds was observed by c ell counting and scanning electr onic microscope.Results Cells adhered and prol iferated on th e three-dimensional scaffolds,and s canning electronic microsco pe show ed the porous structure of the two materials and the eugonic growth of c ells in the scaffolds.Conclusion It is feasible to establish three-di mensiona l cultured model of human periodontal ligament cells by seeding the cells on the scaffolds of CBM or nH AC.And CBM and nHAC are valued to further study as the scaffolds of periodontal tissue engineering.
出处
《中国临床康复》
CSCD
2002年第17期2538-2539,共2页
Chinese Journal of Clinical Rehabilitation
