摘要
目的 :对膜CD14基因进行截短突变和真核表达优化重组 ,以期得到可溶性CD14全长和最小功能段基因。方法 :根据设计合成引物 ,以膜CD14基因为模板 ,PCR扩增目的基因 ,亚克隆入pGEM -T载体 ,进行序列分析。结果 :PCR扩增分别得到 1.1和 0 .5kb的 2个基因片段 ,大小和序列同预期一致。结论 :通过PCR扩增获得重组可溶性CD14全长及最短功能段基因 ,为其结构和功能研究奠定了基础。
AIM:To clone and recombine soluble CD14 gene from membrane CD14 gene. METHODS: Polymerase chain reaction was used to generate soluble CD14 gene, and gene were verified by sequence analysis. RESULTS: Two different sCD14 truncated mutants with 3'ends separately terminating at amino acids 348 and 152 of the mature protein were generated. The sizes of the two mutants are 1.1 kb and 0.5 kb. DNA sequence analysis verified the PCR-generated changes as well as the integrity of the remainder of the CD14 cDNA. CONCLUSION: Soluble CD14 gene was generated by polymerase chain reaction, which could be the foundation for further researches.
出处
《牙体牙髓牙周病学杂志》
CAS
2002年第8期407-409,共3页
Chinese Journal of Conservative Dentistry
基金
军事医学科学院微生物流行病研究所承担国家自然科学基金项目 (3 9870 3 16)
