摘要
As one of near-infrared（NIR） fluorescent（FL） nanoprobes, gold nanoclusters（Au NCs） are delicated to passive-targeting tumors for NIR FL imaging, but which easily cleared by the kidneys for the small size（〈1.5 nm）. Herein, the well-defined gold clusters nanoassembly（Au CNA） was synthesized by the selfassembly of Au NCs based on protein cross-linking approach. The as-prepared Au CNA demonstrated highly effective cellular uptake and precise tumor targeting compared to that of Au NCs. Moreover, with the irradiation of 660 nm laser, Au CNA generated largely reactive oxygen species（ROS） for photodynamic therapy（PDT）. In vitro and [39TD$IF]in vivo PDT revealed that Au CNA exhibited largely cell death and significantly tumor removal at a low power density of 0.2 W/cm^2. It could be speculated that the laser-excited Au CNA produced photon energy, which further obtained electron from oxygen to generate radical species.Therefore, Au CNA as a photosensitizer could realize NIR FL imaging and NIR laser induced PDT.
As one of near-infrared（NIR） fluorescent（FL） nanoprobes, gold nanoclusters（Au NCs） are delicated to passive-targeting tumors for NIR FL imaging, but which easily cleared by the kidneys for the small size（〈1.5 nm）. Herein, the well-defined gold clusters nanoassembly（Au CNA） was synthesized by the selfassembly of Au NCs based on protein cross-linking approach. The as-prepared Au CNA demonstrated highly effective cellular uptake and precise tumor targeting compared to that of Au NCs. Moreover, with the irradiation of 660 nm laser, Au CNA generated largely reactive oxygen species（ROS） for photodynamic therapy（PDT）. In vitro and [39TD$IF]in vivo PDT revealed that Au CNA exhibited largely cell death and significantly tumor removal at a low power density of 0.2 W/cm^2. It could be speculated that the laser-excited Au CNA produced photon energy, which further obtained electron from oxygen to generate radical species.Therefore, Au CNA as a photosensitizer could realize NIR FL imaging and NIR laser induced PDT.
基金
supported by the Major State Basic Research Development Program of China(973 Program)(No.2015CB755500)
National Natural Science Foundation of China(Nos.31571013,21375141,81501580,81401521,81301272 and 81571745)
Shenzhen Science and Technology Program(Nos.KQCX20140521115045447,JCYJ20150403091443298,JCYJ20130402092657771,JCYJ20150401145529015 and JCYJ20160229200902680)
Instrument Developing Project of the CAS(No.YZ201439)
Key International S&T Cooperation Project(No.2015DFH50230)
Guangdong Natural Science Foundation of Research Team(2016A030312006)
