摘要
污蝇杆菌(Wohlfahrtiimonas chitiniclastica)是一类新出现的罕见的人畜共患致病菌。2016年10月,吉林省某肉牛场一头育肥牛眼睑出现化脓病灶,使用多种抗菌药物治疗效果不佳。无菌采集病牛眼睑分泌物,进行细菌分离培养。分离菌株经培养特性、染色镜检、生化试验、16SrRNA序列分析,确定为污蝇杆菌,其对小鼠有致病性。分别用20种抗生素对分离到的菌株使用K-B纸片扩散法测定其耐药性,结果显示对青霉素、头孢拉定、红霉素耐药,对其余17种药物敏感。分别对β-内酰胺类耐药基因(TEM、ADC、OXA-23、OXA-51)、大环内酯类耐药基因(ermA、ermX)以及四环素耐药基因(tetK、tetW)进行检测,结果均显示阴性。
Wohlfahrtiimonas chitiniclastica is a kind of emerging, rare zoonotic pathogen. In October of 2016, a head of fattening cattle from a beef cattle farm of Jilin Province showed appear purulent lesions in the eyelid, and a variety of antimicrobial treatments are not effective. Eyelid secretion of diseased cattle were collected to isolate and culture bacteria. According to culture, staining, biochemical test and 16S rRNA sequence analysis, the isolated strain was identified as Wohlfahrtiimonas chitiniclastica, and it was pathogenic to mice. The resistance of the isolate was determined by K-B disk diffusion method using 20 antibiotics. The results showed that the isolate was resistant to penicillin, cefradine and erythromycin, and it was sensitive to the other 17 drugs. Then the β-lactam resistance gene （TEM, ADC, OXA-23, OXA-51）, macrolide resistance genes （ermA, ermX） and tetracycline resistance genes （tetK, tetW） were detected, respectively, and the results were negative.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2017年第8期1505-1510,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
吉林省现代农业产业技术体系建设专项(2015-24)
吉林省动物疾病防治研究创新团队(20111820)
国家科技支撑计划项目(2014BAK19B005)
吉林省科技成果转化项目(20140309012NY)
关键词
污蝇杆菌
分离鉴定
耐药性
耐药基因
Wohlfahrtiimonas chitiniclastica
isolation and identification
drug resistance
resistance gene
