摘要
目的应用生物信息学方法分析广州地区人巨细胞病毒(human cytomegalovirus,HCMV)临床低传代病毒株D3的UL147基因B细胞抗原表位。方法应用生物信息学方法:用TMHMM预测跨膜区域,用改进型自我优化预测结构(SOPMA)、Garnier-Osguthorpe-Robson(GOR)法、人工神经网络预测法(HNN)预测二级结构,用Hopp&Woods亲水性方案、Janin可及性方案、Welling抗原性方案等参数预测UL147基因抗原表位。结果临床低传代HCMV病毒株D3的UL147基因包含159个氨基酸残基,等电点为9.56;UL147存在一个跨膜区域,为20~42aa区域,胞外区域为1~19aa区域;无规则卷及β-转角区域主要位于12~17、22~28、31~48、99~109、130~145aa区域;亲水性位于15~39、101~111、135~143aa区域;可及性位于14~45、103~124、131~143aa区域;抗原性位于9~14、63~70、108~116aa区域。结论临床低传代HCMV病毒株UL147基因抗原表位可能位于7~12aa区域或其附近。
Objective To analyze the B cell epitopes of the UL147 gene of human cytomegalovirus（HCMV）clinic low passage strain D3 in Guangzhou,China. Methods Bioinformatics method Hidden Markov Model was used to analyze the transmembrane structure.The improved self-optimized prediction method,Garnier-Osguthorpe-Robsonand and Hierarchical neutral network were used to predict the secondary structure of the UL147 gene.The UL147 gene antigenic epitopes were analyzed by Hopp Woods Hydrophilicity Program,Emini Accessibility Program and Jameson-Wolf Antigenicity Program. Results HCMV UL147 contains 159amino acid residues and the isoelectric point was 9.56.The 20-42 amino acids is a transmembrane region and the extracellular region is from 1-19 amino acids,these structures were predicted by TMHMM.The random coil orβ-turnis locates at 12-17,22-28,31-48,99-109 and 130-145 amino acid regions.Parameter prediction revealed that the hydrophilic property locates at 15-39,101-111 and 135-143 amino acid regions;the surface accessibility locates at 14-45,103-124 and 131-143 amino acid regions;the antigenic region locates at 9-14,63-70 and 108-116 amino acids. Conclusions The B cell epitopes of HCMV UL147 gene may locate at or around the region of 7-12 amino acids.
出处
《中国病毒病杂志》
CAS
2017年第3期191-196,共6页
Chinese Journal of Viral Diseases
基金
国家自然科学基金(81070516)
广东省自然科学基金(2016A030313788
2015A030313726)
广东省医学科研基金(A2015133)
