摘要
目的探讨普罗布考对睾丸支持细胞氧糖剥夺/复氧(OGD/R)损伤的影响及机制。方法以原代小鼠支持细胞氧糖剥夺2 h复氧3 h为模型。普罗布考浓度分别为5、10、20μmol/L,复氧同时给药至培养结束。用试剂盒检测细胞增殖活性,用流式细胞术检测细胞活性氧簇(reactive oxygen species,ROS)水平,用试剂盒检测细胞内丙二醛(malondialdehyde,MDA)、铁离子、谷胱甘肽(glutathione,GSH)的含量和谷胱甘肽过氧化物酶(glutathione-dependent peroxidase,GPXs)的活性。结果 Ferroptosis抑制剂可以抑制细胞死亡(P<0.01);普罗布考可降低细胞死亡率(P<0.01),且呈时间与剂量依赖性;普罗布考可以降低细胞内ROS、MDA和铁含量并提高GSH含量和GPXs活性(P<0.05)。结论普罗布考可能通过抑制脂质过氧化介导的ferroptosis发挥对支持细胞损伤的保护作用。
Objective To investigate the effect of probucol on Sertoli cell injury induced by oxygenglucose deprivation/reoxygenation（OGD/R）and mechanism.Methods Primary Sertoli cells treated with oxygen glucose deprivation for 2 h and reoxygenation for 3 h were modeled.We incubated Sertoli cells with probucol in a concentration of 5,10,and 20μmol/L from reoxygenation to the end.The cell viability was evaluated using Cell Proliferation Assay kit.ROS production was observed by flow cytometry.The levels of MDA,iron and GSH or the activity of glutathione-dependent peroxidase were measured by Assay Kit.ResultsThe ferroptosis inhibitors prevented cell death（P〈0.01）.The cell death rate was decreased by probucol in time-and dose-dependent manner（P〈0.01）.Meanwhile,probucol caused increase of ROS,MDA,GSH content and GPXs activity（P〈0.05）.Conclusion Probucol protects Sertoli cells from OGD/R damage via inhibiting lipid peroxidation-mediated ferroptosis.
出处
《实用医学杂志》
CAS
北大核心
2017年第16期2642-2645,共4页
The Journal of Practical Medicine
