摘要
目的:探讨抑制Bmi1表达对舌鳞癌细胞迁移和侵袭的影响。方法:应用Western免疫印迹检测沉默Bmi1后舌鳞癌细胞株UM1细胞(高转移株,UM1 Control siRNA/UM1 Bmi1 siRNA)中侵袭转移相关基因(SOD2、Slug)及干细胞标志物(ABCG2、Nanog)的表达水平。Transwell试验检测沉默Bmi1后UM1细胞的迁移、侵袭能力。球囊形成和平板克隆实验检测沉默Bmi1后UM1细胞的球囊和克隆形成率。CCK8法检测沉默Bmi1后UM1细胞的增殖能力。采用SPSS17.0软件包对数据进行统计学处理。结果 :转染Bmi1 siRNA,使UM1细胞中Bmi1表达水平下调后,UM1细胞的迁移和侵袭能力显著下降;球囊形成率和克隆形成率也显著低于对照组;增殖能力受到抑制;SOD2、Slug和干细胞标志物ABCG2和Nanog的表达水平显著下降。结论:沉默Bmi1可抑制舌鳞癌细胞的迁移和侵袭。
PURPOSE： To investigate the mechanism of Bmil inhibiting migration and invasion of tongue squamous cell carcinoma （TSCC） cell lines. METHODS： Western blot assay was used to detect the expression of invasion and metastasis related genes （SOD2 and Slug） and stem cell markers （ABCG2 and Nanog） in UM1 cells after Broil knockdown. Transwell assay was performed to detect the migration and invasion abilities of UMl cells after Bmil knockdown. Sphere forming assay was performed to detect sphere forming rates of UM1 cells after Bmil knockdown. Colony forming assay was used to detect colony forming rates of UM1 cells after Bmil knockdown. CCK8 assay was used to detect the proliferation ability of UM1 cells after Bmil knockdown. The data were analyzed by SPSS 17.0 software package. RESULTS： After knockdown of Bmil in UM1 cells, the migration and invasion abilities were significantly inhibited compared to control siRNA transfection. UM1 cells transfected with Broil siRNA displayed significantly decreased sphere formation and colony formation compared to the cells transfected with control siRNA. The proliferation of UM 1 cells was significantly inhibited after transfection with Bmil siRNA. The expression of SOD2, Slug and stem cell markers （ABCG2, Nanog） in UMI cells were significantly decreased after transfection with Broil siRNA. CONCLUSIONS： Broil knockdown inhibited the migration and invasion ability of TSCC cell lines.
出处
《中国口腔颌面外科杂志》
CAS
2017年第4期300-304,共5页
China Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金(81472523)
广东省自然科学基金(2015A030313017)
