摘要
本研究旨在探索新疆褐牛产奶性状相关候选基因多态性与泌乳性状及体细胞评分(SCS)的相关性,探究新疆褐牛多态性位点的群体遗传特征。基于课题组前期DNA混池重测序的结果筛选17个SNPs突变信息,并采用基质辅助激光电离解吸飞行时间质谱法(Sequenom Mass Array Genotype)分型技术验证FASN、FSHR、HAL、GPIHBP1、NIBPL、C9、PDE9A、PTK2、STAT1、ZNF66、ZNF567、ZNF7、ZNF623和ZNF804A基因在新疆乌鲁木齐种牛场、新疆天山畜牧生物工程股份有限公司和新疆塔城地区种牛场共计262头新疆褐牛母牛群体中的遗传多态性,运用SAS 8.1软件GLM过程分析这14个基因多个SNP位点对新疆褐牛群体的遗传效应及其与305天产奶量、乳脂率、乳蛋白率、乳糖率、总固体和SCS的关联性。结果表明,17个SNPs均处于哈代-温伯格平衡状态(P>0.05),9个SNPs与新疆褐牛1个或多个泌乳性状达到显著(P<0.005)或极显著(P<0.001)关联,这些位点分别位于FASN、FSHR、HAL、ZNF66、ZNF7、ZNF804A、GPIHBP1和PDE9A基因上。提示这些基因可尝试作为新疆褐牛产奶性状分子标记的候选基因,为新疆褐牛产奶性状的分子标记辅助选育提供依据和参考。
This study aims to explore the association between polymorphisms of candidate genes related to milk production traits and somatic cell score （SCS）, and explore the genetic feature of polymorphism from these SNPs in Xinjiang brown cattle. Totally 262 cattle from Urumqi cattle farm, Tianshan Animal Husbandry Biological Engineering Co., Ltd and Tacheng cattle farm were selected. Based on previous SNPs data obtained by re-sequencing technology in DNA pools, totally 17 SNPs of FASN, FSHR, HAL, GPIHBP1, NIBFL, C9, PDE9A, PTK2, STAT1, ZNF66, ZNF567, ZNF7, ZNF623 and ZNFSO4A genes were screened. At the same time, combining with Sequenom MassArray Genotype technology, the genetic polymorphism was detected. The genetic effect of the 17 SNPs on 305-day milk yield, milk fat percentage, milk protein percentage, laetin percentage, total solids content and SCS were analyzed by GLM procedure of SAS 8.1. The results showed that the 17 SNPs were in Hardy-Weinberg equilibrium, and 9 SNPs had significant （P〈0.005） or extremely significant （P〈0.001） association with milk production traits, and these SNPs were located in FASN, FSHR, HAL, ZNF66, ZNF7, ZNFSO4A, GPIHBP1 and PDE9A genes. The results indicate that these genes can be taken as candidate genes for screening molecular markers of milk production traits. Association analysis of this study provides a theoretical basis and reference for the molecular marker-assisted selection of Xinjiang brown cattle.
作者
刘丽元
周靖航
张梦华
魏趁
王丹
方季青
李金芝
王爱芳
黄锡霞
Liu Liyuan Zhou Jinghang Zhang Menghua Wei Chen Wang Dan Fang Jiqing Li Jinzhi Wang Aifang Huang Xixia(ICollege of Animal Science and Technology, Xinjiang Agricultural University, Urumqi 83005 School of Agriculture, Ningxia University, Yinchuan 750021 Cattle Farm in Urumqi, Urumqi 830000 Cattle Farm in Tacheng Area of Xinjiang, Tacheng Xinjiang 834700 Xinjiang Tianshan Animal Husbandry Biological Engineering Co., Ltd, Changji Xinjiang 831100)
出处
《中国农学通报》
2017年第20期113-123,共11页
Chinese Agricultural Science Bulletin
基金
"十二五"农村领域国家科技计划课题"优质奶牛新品种(系)选育与关键技术研究及示范"(2011BAD28B02)
现代农业(奶牛)产业技术体系建设专项资金资助(CARS-37)
关键词
新疆褐牛
产奶性状
SCS
候选基因
SNP
关联分析
Xinjiang brown cattle
milk production traits
SCS
candidate gene
SNP
association analysis
