摘要
为了研究缩节胺调节棉花对棉蚜酶系活性的影响,本研究测定了取食缩节胺处理后3、6、9、12 d棉叶的棉蚜体内SOD、POD、CAT、GST、ACh E、Car E活性变化。结果表明,棉蚜取食喷施缩节胺的棉花叶片后,3-6 d SOD、POD、CAT的酶比活力均呈现上升趋势,第6 d时,SOD和CAT均达最大值,随后呈下降趋势,POD比活力仅在第6 d时处理组高于对照组,其他均低于对照组。棉蚜体内的GST、Car E在整个取食期间(3-12 d)均为随着取食时间延长而呈下降趋势,均在第12 d达到最低值,其中Car E在不同时间均低于对照,GST却相反。Ach E比活力在棉蚜整个取食期间也均为处理组低于对照组,并且在第12 d降至最低值。这些结果说明缩节胺的处理会使棉蚜产生应激反应,并引起SOD、POD、CAT 3种酶比活力在短时间内增大,但在取食一段时间后有所下降,GST酶在棉蚜的应激反应中发挥了主要作用。结论:缩节胺通过参与棉花的生理代谢过程,诱导棉花增加次生代谢物的合成和积累,从而引起棉蚜保护酶和解毒酶活性的变化;棉蚜的存在增强了棉花的自我防御反应,而缩节胺的处理放大了棉花的这种防御反应。
The objective of this experiment was to study the effect of mepiquat chloride （DPC） on the enzyme activity of wingless cotton aphids （Aphis gossypii Glover）. The activities of superoxide dismutase （SOD）, peroxidase （POD）, catalase （CAT）, glutathione-S-transferase （GST）, acetylcholinesterase （ACHE）, and carboxylesterase （CarE） were measured in cotton aphids fed on cotton leaves that had been sprayed with DPC at 3, 6, 9, and 12 d, respectively. Aphids that fed on untreated （i.e., no DPC） leaves served as the control. The SOD, POD, and CAT activities of the aphids in the DPC group increased between 3 d and 6 d. The SOD and CAT activities reached maximums at 6 d and then declined. The POD activity in the DPC group was significantly higherer than that in the control group at 6 d; however, POD activity in the DPC group was significantly lower than that in the control group on the other sampling days. The GST, ACHE, and Care activities of the aphids decreased during the entire feeding period, reaching minimum values at 12 d. The CarE activities in the DPC group were lower （but not significantly） than those in the control, but GST activity was opposite. The results showed that the treatment of DPC cause stress reaction of cotton aphids, and result in the increasing enzyme activities of SOD, POD, and CAT in a short period of time, but the activities of those enzymes eventually declined. The GST activity plays a major role in the stress response of cotton aphids. In conclusion, may induce the synthesis and accumulation of secondary metabolites through the physiological metabolism of plants, which may lead to changes in protective enzymes and detoxification enzymes. The presence of cotton aphids enhances the self-defense response of cotton, and application of DPC can magnify the defense response of cotton.
作者
钟亮
王巧利
崔家丽
李文杰
王俊刚
Zhong Liang Wang Qiaoli Cui Jiali Li Wenjie Wang Jungang(College of Agriculture, Shihezi University, Shihezi, Xinjiang 832003, Chin)
出处
《石河子大学学报（自然科学版）》
CAS
2017年第2期195-200,共6页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(31260435
31660519)
关键词
棉花
棉蚜
缩节胺
保护酶
解毒酶
cotton
cotton aphid
DPC
protective enzymes
detoxification enzymes
