摘要
目的观察血清Ig G联合IL-5、维生素D检测在肺炎支原体感染合并支气管哮喘患儿中的应用效果。方法选取2014年3月-2016年5月医院诊治的肺炎支原体感染患儿90例,根据是否合并支气管哮喘分为肺炎支原体感染组(n=54)及合并支气管哮喘组(n=36)。选取同期入院健康体检的健康儿30例,设为对照组。采用酶联免疫法(ELISA)法检测血清Ig G、Ig M及Ig E水平;采用酶联免疫检测试剂盒检测人白介素-5(IL-5)水平;采用液相色谱串联质谱检测技术测定维生素D水平。结果合并支气管哮喘组患者血清Ig G、Ig M及Ig E水平,显著高于肺炎支原体感染组和对照组(P<0.05);合并支气管哮喘组IL-5水平,显著高于肺炎支原体感染组和对照组(P<0.05);合并支气管哮喘组维生素D水平,显著低于肺炎支原体感染组和对照组(P<0.05);非条件Logistic多因素分析显示:肺炎支原体感染合并支气管哮喘发生率与血清Ig G联合IL-5、维生素D水平关系密切(P<0.05)。结论肺炎支原体感染合并支气管哮喘患儿治疗过程中检测血清Ig G联合IL-5、维生素D效果理想,值得推广应用。
Objective To observe the effect of serum IgG combined with IL-5 and vitamin D in children with mycoplasma pneumoniae infection and bronchial asthma. Methods 95 children with mycoplasma pneumoniae infection were randomly divided into the mycoplasma pneumoniae group( n = 54) and the bronchial asthma group( n =36) according to whether or not they had bronchial asthma from March,2014 to May,2016. Another 30 healthy children were selected as the control group. The levels of serum IgG,IgM and Ig E were detected by enzyme-linked immunosorbent assay( ELISA). The level of IL-5 was detected by enzyme-linked immunosorbent assay( ELISA) and the level of vitamin D by liquid chromatography-tandem mass spectrometry. Results The levels of serum IgG,Ig M and Ig E in the bronchial asthma group were significantly higher than in the mycoplasma pneumoniae infection group and the control group( P < 0. 05). The level of IL-5 in the bronchial asthma group was significantly higher than that in the mycoplasma pneumoniae group and the control group( P < 0. 05). The unconditional logistic multivariate analysis showed that the incidence of mycoplasma pneumoniae associated with bronchial asthma was significantly correlated with IgG,IL-5 and vitamin D( P < 0. 05). Conclusion The detection of serum IgG,IL-5 and vitamin D has ideal effect in treatment of mycoplasma pneumoniae infection and bronchial asthma,which is worthy to be popularized.
出处
《临床肺科杂志》
2017年第9期1617-1620,共4页
Journal of Clinical Pulmonary Medicine
基金
四川省教育厅重点项目(No 13ZA0216)
