摘要
为研究火龙果茎多糖的超高压提取工艺及抗氧化活性。在单因素试验基础上,采用Box-Behnken设计和响应面分析方法,确定超高压提取的最优工艺,并从清除ABTS自由基和DPPH自由基能力方面来评价火龙果茎多糖的体外抗氧化能力。结果表明,火龙果茎多糖的超高压提取的最佳工艺条件为:液固比10∶1(m L∶g)、超高压压力300 MPa、超高压时间4 min。在此工艺条件下多糖得率为(2.83±0.02)%。火龙果茎对ABTS自由基和DPPH自由基具有清除作用,对ABTS自由基和DPPH自由基的清除率IC_(50)分别为浓度为4.3 mg/m L和5.5 mg/m L时。
To study the technology of ultra high pressure extraction and analysis its antioxidannt activity of polysaccharides from pulp pitaya stem, on the basis of single factor experiments, the optimal processing conditions were determined by means of response surface analysis and Box-Behnken design. The results showed that the optimum extraction conditions of the polysaccharides from pulp pitaya stem were as follows： liquid to solid ratio10 ∶ 1（m L/g）, pressure300 MPa and extraction time 4min. Under the conditions, the extraction yield of the polysaccharides from pulp pitaya stem reached（ 2.83 ±0.02）%. The polysaccharides from pulp pitaya stem had the ABTS and DPPH scavenging activity. The mass concentration of IC_（50） was 4.3 mg/m L and 5.5 mg/m L, respectively. This study could provide useful references for the development and exploitation of the polysaccharides from pulp pitaya stem.
出处
《热带作物学报》
CSCD
北大核心
2017年第7期1371-1376,共6页
Chinese Journal of Tropical Crops
基金
基金项目 河南省食品加工过程安全控制技术创新团队(No.C20150024)
关键词
火龙果茎
多糖
超高压
抗氧化
pulp pitaya stem
polysaccharides
ultra high pressure
antioxidant
