摘要
目的 了解乌鲁木齐市外环境中禽流感病毒的分布情况,为乌鲁木齐市人禽流感防控提供参考依据.方法 采用实时荧光RT-PCR法对收集的外环境标本进行流感病毒A型核酸监测,其中阳性标本进一步作H5、H7、H9亚型的分型检测.监测数据采用SPSS21.0软件进行统计分析.结果 2013-2015年共收集1 043份禽流感外环境标本,核酸检测 FluA 阳性123份,阳性率为11.79%,其中H9亚型阳性检出率最高,为7.77%;阳性检出率高峰每年不同;城乡活禽市场的阳性检出率最高 （14.23%）;禽类饮水和其他标本阳性检出率较高,分别为18.44%和19.44%.结论乌鲁木齐市外环境中常年有H5、H7、H9等多种亚型禽流感病毒,病毒活跃期每年不同,有人感染禽流感的风险,全年均应加强监测.
Objective To investigate the distribution of avian influenza A viruses in external environment in Urumqi City in order to provide reference for the prevention and control of human infection with avian influenza virus in Urumqi City.Methods Environmental specimens were collected from 2013 to 2015 to detect avian influenza A virus nucleic acid with real-time fluorescent PCR.The positive specimens were further analyzed to identify the subtypes of avian influenza A viruses.Results A total of 1 043 environmental specimens were collected and tested.Among them,123 specimens were positive for avian influenza A virus nucleic acid with an overall positive rate of 11.79%.H9 was the predominant subtype,accounting for 7.77% of all specimens.Quarterly detection rates of avian influenza A virus in the three years peaked in different quarters of the year.Of all surveillance sites,live poultry markets in urban and rural areas had the highest positive rate （14.23%）.Specimens collected from poultry drinking water （18.44%） and other specimens （19.44%） were highly positive for avian influenza A virus.Conclusion Avian influenza A viruses,especially the subtypes of H5,H7 and H9,exist in live poultry markets in Urumqi City.It is necessary to conduct surveillance and health education among people exposed to poultry in live poultry markets.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2017年第6期457-460,共4页
Chinese Journal of Microbiology and Immunology
关键词
城乡活禽市场
外环境
禽流感病毒
Urban and rural live poultry market
External environment
Avian influenza virus
