摘要
目的通过体内和体外实验观察氧化石墨烯纳米颗粒的急性毒性作用。方法选择60只健康ICR小鼠,雌雄各半,随机分成6组,每组10只,以高纯水配制纳米氧化石墨烯,分别以剂量0 mg/(kg·bw)、3.125 mg/(kg·bw)、6.25 mg/(kg·bw)、12.5 mg/(kg·bw)、25.0 mg/(kg·bw)和50.0 mg/(kg·bw)进行纳米氧化石墨烯灌胃,观察动物一般状态及死亡情况;另取60只ICR小鼠,分为6组,在无菌条件下,以高纯水配制纳米氧化石墨烯,以剂量0 mg/(kg·bw)、1.0 mg/(kg·bw)、2.0 mg/(kg·bw)、4.0 mg/(kg·bw)、8.0 mg/(kg·bw)和16.0 mg/(kg·bw)进行尾静脉注射,观察动物一般状态及死亡情况。采用MTT法,以剂量为9.875 mg/(kg·bw)、18.75 mg/(kg·bw)、37.5 mg/(kg·bw)、75 mg/(kg·bw)、150 mg/(kg·bw)、300 mg/(kg·bw)和600 mg/(kg·bw)的氧化石墨烯与A549细胞进行共培养,观察细胞的生长状况并计算细胞的LC_(50)。结果纳米氧化石墨烯经口染毒途径未引起小鼠器官病理改变,无动物死亡;通过尾静脉注射对小鼠的LD_(50)为5.657 mg/(kg·bw),肺、肝、心组织血管内可见褐色纳米颗粒,但组织未见病理学变化;对A549细胞的LC_(50)为56.885μg/m L。结论未经修饰的纳米氧化石墨烯经尾静脉注射可导致动物死亡,并对A549细胞生长具有抑制作用。
Objective To observe the acute toxicity of nano grapehene oxide on mice in vitro and in vivo. Methods Sixty healthy ICR mice, half male and half female, were divided into six groups and treated with nano graphene oxide dissolved in ultrapure water by intragastric administration at the dosage of 0, 3. 125 mg/（kg·bw） , 6.25 mg/（ kg·bw）, 12.5 mg/（ kg· bw）, 25.0 mg/（ kg· bw） and 50.0 mg/（ kg· bw） respectively. Another 60 ICR mice were treated with nano graphene oxide dissolved in sterile pure water at the dosage of 0, 1.0 mg/（ kg· bw） , 2.0 mg/（ kg· bw） ,4.0 mg/（ kg· bw） , 8.0 mg/（ kg· bw） and 16.0 mg/（ kg· bw） respectively by intravenous injection under aseptic condition. Meanwhile, A549 cells were co-cuhured with nanograpehene oxide at the concen- trations of 0, 9. 875 mg/（ kg· bw）, 18.75 mg/（ kg· bw）, 37.5 mg/（ kg· bw）, 75 mg/（ kg· bw）, 150 mg/（ kg· bw） , 300 rag/（ kg· bw） , and 600 mg/（ kg· bw） respectively. Results No pathological change of main organs had been observed in mice caused by intragastric administration of nano graphene oxide, and the LDso was 5. 657 mg/ （kg-bw） of nano graphene oxide for mice caused by caudal vein injection. The LCs0 of nano graphene oxide for A549 cell was 56. 885 μg/mL. Conclusion Intravenous injection of unmodified nano graphene oxide could cause mice death and the existence of nano graphene oxide in culture medium could inhibit the survival of A549 cell.
作者
刘艳
于杨
陈杰
岳美
马荣爽
赵淑华
LIU Yan1 YU Yang2 CHEN Jie2 YUE Mei2 MA Rongshuang2 ZHAO Shuhua2
出处
《环境卫生学杂志》
2017年第3期233-238,共6页
JOURNAL OF ENVIRONMENTAL HYGIENE
