摘要
利用ISSR分子标记技术对60份丝瓜种质资源进行遗传多样性分析,100对ISSR引物中筛选出12对引物,共扩增条带154条,平均每个引物扩增条带14条,多态性条带143条,非多态性条带11条。各个引物的多态性比例范围为50%~100%,多态性比例92.9%。结果表明,丝瓜种质资源遗传多样性较高,同时也表明了ISSR分子标记能够很好的检测丝瓜种质资源的遗传多样性。通过聚类分析将60份丝瓜分为2大类,普通丝瓜和棱丝瓜。分别以0.646和0.718处为阈值,普通丝瓜和有棱根据瓜形分为短圆筒普通丝瓜、长圆筒普通丝瓜、短棍棒有棱丝瓜和长棍棒有棱丝瓜。
60 Luffa cultivars were molecularly characterized with ISSR markers. 12 pairs of ISSR primers were screened out of 100 ISSR primer pairs which produced 154 DNA bands, the average number of polymorphic DNA bands amplified by each primer pair was 14, polymorphic bands 143, basic bands 11, the proportion of polymorphic primers ranged from 50% to 100% and the ratio of polymorphic bands was 92.9%. The results showed the high genetic diversity of Luffa germplasm resources. ISSR could be used to detect genetic diversity of germplasm resources of Luffa. The 60 Luffa germplasm were clustered into 2 groups based on the morphological data. The first group was Luffa cylindrica（L.） M.J. Roem with two subgroups including short cylinder and long cylinder types, and the second group was Luffa acutangula（L.） Roxb. with two subgroups including short sticks and Long sticks types, classified with the similarity coemcient of 0.646 and 0.718.
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第6期2450-2456,共7页
Molecular Plant Breeding
基金
福建省属公益类科研院所基本科研专项(2017R1026-6)
福建省农业科学院创新团队PI项目(2016PI-40)
福建省自然科学基金项目(2015J01118)
福建省种业创新与产业化工程(FJZZZY-1528)共同资助
