摘要
目的评估载脂蛋白C-Ⅲ(Apolipoprotein,ApoC-Ⅲ)在溃疡性结肠炎(UC)的诊断及鉴别诊断中的作用。方法收集病理科2003~2011年存档的肠组织标本,UC组38例,克罗恩病(CD)组29例,肠结核组31例,肠淋巴瘤组32例,感染性肠炎组32例,白塞病组28例,正常体检组10例,采用免疫组化检测结肠黏膜的ApoC-Ⅲ表达情况,并用图像分析技术计数切片的阳性细胞数,取光学显微镜下的平均阳性细胞数作为计数标准。结果 UC患者肠粘膜组织中ApoC-Ⅲ的表达(44.44±10.71)显著高于肠结核组(20.76±4.77)、克罗恩病组(18.80±3.60)、肠淋巴瘤组(18.83±3.79)、白塞病组(19.02±3.04)、感染性肠炎组(20.56±3.35)和正常组(30.17±6.27,均P<0.05);正常组(30.17±6.27)又高于肠结核组(20.76±4.77)、克罗恩病组(18.80±3.60)、肠淋巴瘤组(18.83±3.79)、白塞病组(19.02±3.04)和感染性肠炎组(20.56±3.35,均P<0.05);但其它5组之间无差异(P>0.05)。结论 ApoC-Ⅲ在UC患者肠粘膜组织的表达较肠结核组、克罗恩病组、肠淋巴瘤组、白塞病组、感染性肠炎组和正常对照组明显增强,提示ApoC-Ⅲ与UC关系密切,可能与UC的发生发展过程相关,并在UC的诊断和鉴别诊断中起关键作用。
Objective To evaluate the role of ApoC-Ⅲ in the diagnosis and differential diagnosis of UC. Methods Intestinal tissue samples were retrieved from 2003-2011 archived specimen at Department of Pathology, and assigned to UC group （n=38）, CD group （n=29）, ITB group （n=31）, IL group （n=32）, infectious enteritis group （n=32） and Behcet＇s disease group（n=28）. 10 cases were recruited as normal control group. The expression of ApoC-± in colonic mucosa were detected by immunohistochemistry. Positive cells were counted by Image Analysis. Results The expression rate of ApoC-Ⅲ in intestinal mucosa of UC patients（44.44±10.71）was significantly higher than that of ITB（20.76±4.77）, CD （18.80±3.60） , IL（18. 83±3. 79） , Behcet＇s disease（19.02±3.04）, Infectious enteritis （20.56＋3.35） and normal control（30. 17±6.27）（p〈0.05）. That of normal control（30. 17±6.27） was higher than ITB（20. 76±4. 77） , CD（18.80±3.60） , IL（18.83±3.79） , Behcet＇s disease（19.02±3.04） , Infectious enteritis（20.56±3.35） （p〈0.05）. While there was no significant difference among the other five groups（p〈0.05）. Conclusion ApoC-Ⅲ is related with UC closely, and may play an imoortant role in the !oathogenesis, diagnosis and differential diagnosis of UC.
出处
《西部医学》
2017年第7期908-911,915,共5页
Medical Journal of West China
基金
国家自然科学基金(81160055/H0310)
