摘要
分别以玉米秸秆、小麦秸秆和杨树木屑为原料,研究了一株密孔菌在发酵不同农业废弃物时漆酶的活性.结果表明,农业废弃物对密孔菌产漆酶具有良好的诱导效果,且以玉米秸秆的效果最佳,漆酶酶活可达40.92 U·mg^(-1).Cu^(2+)的存在或浓度变化显著影响密孔菌产酶,当添加0.02%Cu^(2+)时,菌株在小麦秸秆和木屑培养基上产酶活分别达到41.24 U·mg^(-1)和57.13 U·mg^(-1);当Cu^(2+)浓度为0.04%时,菌株在玉米秸秆培养基上分泌漆酶酶活达到峰值94.92 U·mg^(-1).p H值对密孔菌产漆酶有明显影响,在中酸性环境中菌株产漆酶酶活较高.菌株在小麦秸秆和玉米秸秆培养基上发酵产酶的最适温度较高,分别为35℃和40℃,且在整个发酵周期能维持较高较稳定的酶活水平.因此,密孔菌有很好产漆酶和降解农作物秸秆的能力,可用于农作物秸秆处理.
The aim of this study is to investigate laccase production by Pycnoporus in solid-stage fermentation using different agricultural residues,such as corn stalk,wheat straw and poplar sawdust. The results reveled that agricultural residues can induce Pycnoporus to produce laccase and corn stalk showed the highest effect with production of 40. 92 Umg^（-1）. Moreover,the concentration of Cu^（2＋） has a significant effect on laccase production and the production could reach 41. 24 Umg protein-1 and 57. 13Umg^（-1）using wheat straw and poplar sawdust as medium with 0. 02% Cu^（2＋） ; the production of laccase could reach 94. 92 U·mg^（-1）using corn stalk as medium with 0. 04% Cu^（2＋） . Lassase production was affected by p H obviously and the activity of laccase was higher in neutral and acid environment. The optimum temperature for Pycnoporus to produce laccase in wheat straw medium and corn stalk medium was 35° C and 40° C,respectively. Moreover,laccase activity could maintain a high and stable level during the whole fermentation period. The results from this study indicate that Pycnoporus has a high efficacy in producing laccase and degrading crop straw,which can be used in the treatment of crop straw.
作者
李秀男
牛子琪
胡卫成
王新风
LI Xiu-nan NIU Zi-qi HU Wei-cheng WANG Xin-feng(College of Life Sciences, Huaiyin Normal University, Huaian Jiangsu 223300, China Huaian Key Laboratory of Food Components and Functional Foods, Huaian Jiangsu 223300, China Jiangsu Collaborative Innovation Center of Regional Modern Agriculture ＆ Environmental protection, Huaian Jiangsu 223300, China Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaian Jiangsu 223300, China)
出处
《淮阴师范学院学报（自然科学版）》
CAS
2017年第2期147-153,共7页
Journal of Huaiyin Teachers College;Natural Science Edition
基金
江苏省大学生创新创业训练计划项目(201510323013Z)
江苏高校品牌专业建设工程资助项目(PPZY2015A018)
江苏省农业自主创新基金项目(CX(13)3026)
关键词
密孔菌
固态发酵
农业废弃物
漆酶
pycnoporus
solid state fermentation
agricultural wastes
laccase
