摘要
目的合成靶向表皮生长因子受体(EGFR)壳聚糖吉西他滨纳米粒,研究其在体外胰腺癌细胞的靶向性及对细胞增殖的影响。方法采用壳聚糖制备EGFR-吉西他滨-壳聚糖纳米粒(G-GC-Dox)、吉西他滨-壳聚糖纳米粒(GC-Dox)。在体外实验研究中将药物作用于EGFR+胰腺癌细胞系SW1990细胞,研究胰腺癌细胞体外摄取实验,并采用四甲基偶氮唑蓝法(MTT)法观察该体系对胰腺癌SWl990细胞生长增殖的影响。结果 EGFR-吉西他滨-壳聚糖纳米粒组体外SW1990胰腺癌细胞平均摄取纳米药物的量明显强于同一时间点吉西他滨-壳聚糖纳米粒组平均摄取纳米药物的量。G-GC-Dox组处理12、24、48h后细胞存活率(43.14%±2.51%、31.21%±2.37%、18.26%±2.75%)对比GC-Dox组(64.22%±3.11%、45.43%±3.04%、35.23%±3.15%)对肿瘤细胞具有更好的抑制作用(P<0.05)。结论本实验成功研制了靶向EGFR壳聚糖吉西他滨纳米粒,并证实了该纳米粒能提高药物在体外胰腺癌细胞的靶向性,同时对胰腺癌SW1990细胞增殖具有明显抑制作用,可能为将来靶向治疗胰腺癌提供新的研究思路。
Objective Synthesis of epidermal growth factor receptor （EGFR） gemcitabine chitosan nanoparticles. To study the tar- geting effect of pancreatic cancer cells in vitro and cell proliferation. Methods EGFR - gemcitabine - chitosan nanoparticles（ G - GC - Dox） and gemcitabine- chitosan nanoparticles（ GC- Dox） were prepared by emulsion polymerization method. In vitro immunoflnores- cence was used to study the intake test of EGFR^＋ carried SW1990 cells in G - GC - Dox and GC - Dox. Their ability to inhibit the prolif- eration of SW1990 cell lines by methylthiazolyl tetrazolium （MTT） were also tested. Results Compared to GC - Dox,G - GC - Dox had a better drug concentration in SW1990 cell. The survival rates of SW1990 cells were（43.14% ± 2.51% ,31.21% ± 2.37% , 18.26% ± 2.75% ） when treated with G - GC - Dox after 12h,24h,48h, the survival rate of SW1990 cells were （64.22% ± 3.11% ,45.43% ± 3.04% ,35.23% ± 3.15% ） when treated with GC - Dox. The differences had statistical significance（P 〈 0.05 ）. Conclusion G - GC - Dox was successfully developed. The nanoparticles could improve drug targeting of pancreatic cancer cells in vitro and enhance the antitu- mor of pancreatic cancer. In future, it May provide a new train of thought for the treatment of pancreatic cancer.
出处
《医学研究杂志》
2017年第6期84-87,共4页
Journal of Medical Research
基金
浙江省医药卫生科技计划项目(2013KYB248)
浙江省温州市医药卫生科研项目(2016A02)
浙江省温州市公益性科技计划项目(Y20160527)
关键词
胰腺肿瘤
增殖
吉西他滨
壳聚糖
Pancreatic neoplasms
Proliferation
Gemcitabine
Chitosan
