摘要
目的研究RNAi沉默畸胎瘤细胞源性生长因子(PCDGF)表达对喉鳞癌Hep-2细胞增殖、凋亡及侵袭能力的影响。方法 RT-PCR检测喉鳞癌组织中PCDGF的mRNA表达水平;Hep-2细胞分为空白对照组、阴性对照组和PCDGF-siRNA组,Western blot检测转染效果及Bcl-2、Bax、E-cadherin和MUC1蛋白表达;CCK8实验检测细胞增殖;流式细胞仪检测细胞凋亡;Transwell小室检测细胞侵袭能力。结果喉鳞癌组织中的PCDGF mRNA表达水平显著高于声带息肉组织(t=6.88,P=0.005)。PCDGF在喉鳞癌组织中高表达。PCDGF-siRNA组喉鳞癌Hep-2细胞中PCDGF、Bcl-2、MUCI蛋白表达显著低于空白对照组(P<0.05),Bax、E-cadherin蛋白表达显著高于空白对照组(P<0.05),细胞增殖率显著低于空白对照组(P<0.05),细胞侵袭数显著低与空白对照组(P<0.05),细胞凋亡率显著高于空白对照组(P<0.05)。结论抑制PCDGF的表达能够抑制喉鳞癌Hep-2细胞的增殖和侵袭能力,诱导细胞发生凋亡。
Objective To explore the effects of PCDGF on proliferation, apoptosis and invasion of laryngeal squamous cell carcinoma Hep-2 cells.Methods The mRNA expression of PCDGF in laryngeal squamous cell carcinoma was detected by RT-PCR;Hep-2 cells were divided into blank control group, negative control group and PCDGF-siRNA group;Bcl-2, Bax, E-cadherin and MUC1 protein expression were detected by Western blot;cell proliferation was detected by CCK8 assay;cell apoptosis was assessed by flow cytometry;cell invasion was determined by Transwell assay.Results PCDGF was highly expressed in laryngeal squamous cell carcinoma（t=6.88, P=0.005）.PCDGF, Bcl-2 MUCI protein expression in laryngeal squamous cell carcinoma in PCDGF-siRNA group was significantly lower than that of the control group（P〈0.05）,Bax and E-cadherin protein expression was significantly higher than that of control group（P〈0.05）,cell proliferation rate was significantly lower than that of the blank control group （P〈0.05）,the number of cell invasion was significantly smaller than that of the blank control group（P〈0.05）, and the apoptosis rate was significantly higher than that of the control group （P〈0.05）.Conclusion Inhibiting the expression of PCDGF can block the proliferation and invasion of laryngeal squamous cell carcinoma Hep-2 cells and induce apoptosis.
出处
《安徽医学》
2017年第6期721-725,共5页
Anhui Medical Journal
关键词
畸胎瘤细胞源性生长因子
喉鳞癌
RNAi
Teratoma cell derived growth factor
Laryngeal squamous cell carcinoma
RNA interference
