摘要
目的:探讨心室肌细胞外基质(ECM)对植入大鼠心梗组织内的心肌球源性心肌干细胞(CDC)分化、定植及存活的影响。方法:取雌性Wistar大鼠建立急性心梗模型,并分别采用心肌组织块法和脱细胞法制备CDC和ECM。IC组(n=6)在依斯考夫改进的杜尔贝科培养基(IMDM)中加入CDC进行培养,培养终浓度为1×106·(150μl)-1;EC组(n=6)在ECM中加入CDC进行培养,培养终浓度为1×106·(150μl)-1。24 h和3周后测定两组的血管内皮细胞(v WF)、心肌细胞(ɑ-SA)、平滑肌细胞(ɑ-SMA)蛋白表达情况。结果:IC组心肌组织ɑ-SA、v WF、ɑ-SMA的灰度比,心梗后24 h及3周CT值(SRY基因)检测结果与EC组比较,差异均有统计学意义(P<0.05)[分别(0.46±0.06)vs.(0.83±0.03)、(0.15±0.08)vs.(0.31±0.01)、(0.18±0.11)vs.(0.5±0.07)、(1.89±0.67)vs.(5.58±1.44)、(0.5±0.26)vs.(2.18±1.28)]。结论:心肌组织源性的ECM能够促进植入心梗组织内的CDC向心肌、内皮及平滑肌细胞分化,促进心梗后植入CDC的定植及存活。
[ Abstract] Objective： To investigate the effect of extracellular matrix （ECM） on the differentiation, colonization and survival of cardiac muscle derived stem cells （CDC） in rats with acute myocardial infarction. Methods： Female Wistar rats were used to establish acute myocardial infarction model and the CDC and ECM were prepared by the method of myocardial tissue block and cell free. The CDC in IC group （ n = 6） were cultured with Toure Beko medium （IMDM） modified by Iscoves, and the final concentration of culture was 1×10^6·（150μl）^-1 The CDC in EC group （n=6） were cultured within medium, and the final concentration of culture was 1×10^6·（150μl）^-1. The expressions of vascular endothelial cell （vWF）, myocardial cells （α-SA）, smooth muscle cells （α-SMA） of the two groups after 24 h and 3 weeks of implantation CDC were detected. Results： The gray ratio of α- SA, VWF and α- SMA gray ratio, CT value of 24 h, 3 weeks after myocardial infarction （ SRY gene） in the IC group were significantly different from those in the EC group （ P 〈 0.05 ） [ （ 0.46 ± 0.06 ） vs. （0.83 ± 0.03 ）, （0.15 ±0.08） vs. （0.31 ±0.01）,（0.18±0. 11）vs. （ 0.5 ±0.07）,（1.89±0.67） vs. （5.58±1.44）,（0.5 ± 0.26）vs. （2.18 ±1.28）,respectively]. Conclusion： Myocardial tissue derived ECM can promote the differentiation of CDC in myocardium, vascular endothelial cells and smooth muscle cells and promote the implantation and survival of CDC after myocardial infarction.
出处
《现代医学》
2017年第5期627-630,共4页
Modern Medical Journal
基金
国家自然科学基金资助项目(81400196)
关键词
细胞外基质
心肌球源性心肌干细胞
分化
定植
存活
extracellular matrix
cardiosphere- drived cells
differentiation
retention
survival
