摘要
[目的]探讨亚砷酸钠对雄性大鼠血清中炎症因子和氧化应激水平的影响。[方法]将32只健康无特定病原级雄性SD大鼠按体重随机分为4组,分别为对照组(超纯水)和低(10μg/L)、中(100μg/L)、高(1 000μg/L)剂量亚砷酸钠溶液染毒组。每组8只,自由饮水染毒28 d。采用酶联免疫吸附试验测定大鼠血清中粒细胞-巨噬细胞集落刺激因子(GM-CSF)、白细胞介素-10(IL-10)、还原型烟酰胺腺嘌吟二核苷酸磷酸(NADPH)氧化酶、活性氧簇酶(ROS)、总抗氧化能力和一氧化氮(NO)水平。[结果]亚砷酸钠染毒组大鼠血清GM-CSF表达量显著高于对照组(P<0.05),且1 000μg/L组的GM-CSF水平最高;抑炎因子IL-10含量均低于对照组(P<0.05);1 000μg/L剂量组ROS表达水平高于对照组(P<0.05);与对照组相比,各染毒组NO表达量升高(P<0.05),且存在剂量-反应关系。[结论]亚砷酸钠进入雄性大鼠机体后产生炎症损伤和氧化应激损伤,损伤机制可能与NO增多、IL-10降低有关。
[ Objective ] To investigate the effects of sodium arsenite on serum inflammatory cytokine and oxidative stress levels in male rats. [ Methods ~ Thirty-two specific-pathogen-free healthy male SD rats were randomly divided into four groups： control group , low dose, medium dose, and high dose groups, with eight rats in each group. The three exposure groups were intoxicated by 10, 100, and 1 000μg/L NaAsO2 in drinking water, respectively, while the control group was given ultrapure water for free drinking. All the rats were sacrificed after 25 days. The levels of granulocyte-macrophage colony stimulating factor （GM-CSF）, interleukin-lO （IL-IO）, nicotinanmide adenine dinucleotide phosphate （NADPH） oxidase, reactive oxygen species （ROS）, total antioxidant capacity, and nitric oxide （NO） were determined by enzyme-linked immunosorbent assay. [ Results ] Sodium arsenite exposed rats showed significantly higher GM-CSF expression levels than the control group （P〈O.05）, and the 1 000 μg/L group had the highest GM-CSF expression level The anti-inflammatory cytokine IL-IO levels were lower in all exposure groups than in the control group （P 〈0.05）. The 1000 μg/L group showed a higher ROS level than the control group （P〈0.05）. NO expression levels were increased in all exposure groups compared with the control group （P〈 0.05）, and there was a dose-response relationship. [ Conclusion ] Sodium arsenite exposure in male rats could lead to inflammatory injury and oxidative stress injury, and the mechanism may be related to elevated NO and reduced IL-IO.
作者
杨欢
王素华
苏鑫
高艳荣
YANG Huan WANG Su-hua SU Xin GAO Yan-rong(School of Public Health, Baotou Medical College, Baotou, Inner Mongolia 014040, China）.)
出处
《环境与职业医学》
CAS
CSCD
北大核心
2017年第6期542-544,共3页
Journal of Environmental and Occupational Medicine
基金
国家自然科学基金项目(编号:81660532)
内蒙古科技计划项目(编号:201502080)
关键词
砷
亚砷酸钠
炎症因子
氧化应激
arsenic
sodium arsenite
inflammatory cytokine
oxidative stress
