摘要
目的探讨早期补救卵泡浆内单精子注射(ICSI)在常规体外受精(IVF)中完全不受精或低受精的临床应用价值。方法回顾性分析2013年3月~2015年5月在本院生殖中心常规IVF治疗受精6h后判断为完全不受精或低受精而行早期补救ICSI的52周期患者,统计其实验室培养指标及临床结局,并与同期因男方因素而直接进行ICSI的127周期进行比较。结果早补救ICSI组的受精率、卵裂率、临床妊娠率及种植率和直接ICSI组比较差异无统计学意义(P>0.05),而优胚率、胚胎利用率及囊胚形成率显著低于直接ICSI组,两组差异有统计学意义(P<0.05)。结论早补救ICSI可作为常规IVF受精失败的补救措施具有较高的临床应用价值,但由于卵母细胞在体外的培养时间延长导致卵子老化,甚至会影响胚胎的发育潜能。建议对高度怀疑受精障碍尤其高龄(≥37岁)、获卵数≥8患者采用Half-ICSI以提高胚胎利用率和累积妊娠率。
Objective： To study clinical value of early rescue intracytoplasmic sperm injection （ICSI） in conventional vitro fertilization and embyo transfer （IVF） with complete failed fertilization or low fertilization. Methods： The data of 52 cycles from March 2013 to May 2015 were restrospctive analyzed, Early rescue ICSI were performed in cycles with complete failed fertilization or low fertilization after 6h co-incubation of gametes. A total of 127 ICSI patients with male factors infertility served as a control group. The parameters including the rate of fertilization, cleavage, clinical pregnancy and the implantation rate. Results： There were no statistically significant difference in the rate of fertilization, cleavge, clinical pregnancy, implantation （P〉0.05） , high embyo quality rate, the available embryos rate, and blastulation rate in Resue ICSI were low than the group which were ICSI, there were statistically significant difference between the two groups. Conclusion： Early rescue ICSI should be considered in the presence of fertilization failure in IVF. Oocytes would be ageing with the longer culture in vitro, and which can influence the embryo growth potential. The pantients who are old （≥37year） , the number of oocytes retrieve larger than 8 can perform Half- ICSI to improve the available embryos rate and rate of cumulative pregnance.
出处
《中国优生与遗传杂志》
2017年第6期118-120,共3页
Chinese Journal of Birth Health & Heredity
关键词
体外受精
受精失败
第二极体
补救ICSI
In vitro fertilization
Fertilization failure： The second polar body
Rescue ICSI
