摘要
为研究伪狂犬病病毒(PRV)的致病机制,将PRV QBA株按0.5个感染复数(MOI)的剂量接种PK-15细胞,分别在感染后0、1、2、4、6、8、12、24 h收取细胞并提取microRNA(miRNA),采用茎环引物实时荧光定量PCR(RT-q PCR)检测prv-miR-LLT11a的表达时相。RT-q PCR扩增结果显示,熔解曲线峰值单一,扩增曲线拐点清晰。RT-q PCR检测结果表明,PRV QBA株感染PK-15细胞1 h后,prv-miR-LLT11a表达量极显著升高,随后表达量下调,在感染6 h后表达量降至最低,感染8 h后表达量持续急剧升高。
In order to study the pathogenic mechanism of pseudorabies virus （PRY） , PK-15 cells were in -fected with PRY QBA strain at 0. 5 M0I,and cells were harvested and microRNA（ miRNA） were extrac-ted at 0,1 ,2 ,4,6,8,12 ,24 hours post infection（ hpi）. Then the temporal expression of prv-miR-LLTl 1 a was detected by stem-loop real-time quantitative PCR （ RT-qPCR） . The amplification results of RT-qPCR showed that the melting curve had a single peak and a clear inflection point. RT-qPCR data showed that the expression of prv-miR-LLTl 1 a remarkably significant increased in PK-15 cells after 1 hour infection with PRY QBA strain, then decreased. Its expression level decreased to the lowest level at 6 hpi, and in-creased significantly after 8 hpi.
出处
《河南农业科学》
CSCD
北大核心
2017年第6期120-124,共5页
Journal of Henan Agricultural Sciences
基金
国家自然科学基金项目(31272567)
河南省高校科技创新人才支持计划项目(14HASTIT022)
河南省高校科技创新团队支持计划项目(14IRTSTHN015)
