摘要
目的:观察在中药"丹红通精方"干预下,精索静脉曲张不育症患者精液质量变化及缺氧诱导因子-1α(HIF-1α)的表达。方法:将60例精索静脉曲张不育症(气虚血瘀证)患者随机分成两组,每组30例,治疗组予口服丹红通精方,手术组行显微镜下精索静脉低位结扎术。观察两组治疗前后精子浓度、精子总活力、精子前向运动率(a+b级)、精子低渗肿胀率、精子顶体酶、血清及精浆HIF-1缺氧诱导因子-1α水平。结果:治疗前后两组组内比较,精子浓度、精子总活力、精子前向运动率、精子低渗肿胀率、精子顶体酶差异均有统计学意义(P<0.01);精浆HIF-1缺氧诱导因子-1α值比较,差异有统计学意义(P<0.05);血清HIF-1缺氧诱导因子-1α值比较,差异无统计学意义(P>0.05);治疗后组间比较,精子低渗肿胀率差异有统计学意义(P<0.05),精子浓度、精子总活力、精子前向运动率、精子顶体酶、精浆HIF-1α值、血清HIF-1α值差异无统计学意义(P>0.05)。结论:丹红通精方能改善气虚血瘀型精索静脉曲张不育症患者精子浓度、精子总活力、精子前向运动率、精子低渗肿胀率、精子顶体酶,降低精浆HIF-1α,其作用机制可能与改善生殖系统缺氧状态有关。
Objective： To observe the effect of Danhong Tongjing Formula （丹红通精方, DHTJF） and its ef- fect on semen quality changes and expression of HIF-1α. Methods： The 60 patients with varicocele sterility （syndrome of Qi deficiency and blood stasis） were randomly divided into two groups, each group of 30 patients; treatment group was treated with DHTJF, the surgical group was treated with low ligation of spermatic vein un- der the microscope. The sperm concentration, total energy and forward movement of sperm sperm rate （a＋b lev- el）, hypoosmotic swelling rate of sperm, sperm acrosome enzyme, serum and seminal plasma HIF-1αwere detect- ed before and after treatment. Results： Sperm concentration, sperm total energy and forward movement of sperm, sperm acrosin in sperm hypoosmotic swelling rate in both groups all changed after treatment （P〈0.01）; HIF- 1α value in seminal plasma has significant difference （P〈0.05）; while the serum HIF-lothas no significant differ- ence （P〉0.05）. The hypoosmotic swelling rate of sperm between the two groups has significant difference （P〈0.05）; while sperm concentration, total energy and forward movement of sperm, the sperm acrosin, aeminal plasma, serum HIF-1αvalue have no significant differences （P〉0.05）. Conclusion： DHTJF can rectify varicocele infertility sperm concentration, total energy and forward movement of sperm rate, hypoosmotic swelling rate of sperm, and the sperm acrosin of the patients, and reduce refining HIF-1αand its mechanism may be related to improve the state of hypoxia in the reproductive system.
出处
《中医药导报》
2017年第10期72-74,78,共4页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
广东省中医药管理局项目(20151094)
项目名称:中药对精索静脉曲张不育症患者疗效及HIF-1α的影响
