摘要
目的建立了哌嗪类新精神活性物质定性定量分析方法。方法采用安捷伦三重串联四极杆液质联用,Waters Acquity UPLCBEH Pheny1 C18色谱柱(2.1mm×150mm,1.7μm),流动相为0.1%甲酸:乙腈=95%:5%,梯度洗脱,流速为0.5mL/min。质谱应用ESI源、正离子模式、多反应监测(MRM)模式。结果 8种哌嗪类新精神活性物质在1~5000 ng/mL质量浓度范围内线性关系良好,相关系数(r^2)均大于0.992,日内与日间保留时间和峰面积的相对标准偏差在0.56%~7.42%之间,检出限在0.10~0.30ng/mL(S/N≥3)。结论本方法定性定量效果好,线性范围广,精密度高,操作简便,能够在同一实验条件下实现对8种哌嗪类新精神活性物质的有效检出。
Objective A qualitative and quantitative analysis for piperazine-categorical new psychoactive substances was established by LC-MS/MS. Methods Agilent’s Triple Quadrupole LC/MS was used along with Waters’ Acquity UPLC？ BEH Phenyl Cl8 column （2.1mm×150mm, 1.7μm）, which was eluted by the mobile phase （0.1% formic acid： acetonitrile=95%： 5%） at flowing rate of 0.5mL/min with a gradient. Mass spectrometry was carried out with ESI source, plus positive ion mode and multiple reaction monitoring （MRM）. Results This measurement was of good linearity with the real value among the range of 1 - 5000ng/mL. Correlation coefficients （ r2） of all calibration curves were greater than 0.992. Both intra- and inter- day precisions were expressed by relative standard deviations of both retention time and peak area, showing within 0.56%- 7.42%. The detection limit spanned across 0.10-0.30ng/mL （S/N≥3）. Conclusion The method is of good qualitative and quantitative effect, wide linear range, high precision and easy operation, capable of effectively detecting eight new compounds of piperazine under the same experimental conditions.
出处
《刑事技术》
2017年第3期195-198,共4页
Forensic Science and Technology
基金
公安部技术研究计划项目(No.2015JSYJB04)
关键词
哌嗪类新精神活性物质
LC-MS/MS
定性定量
LC-MS/MS
piperazine-categorical new psychoactive substances
qualitative and quantitative
