摘要
目的:观察红景天对支气管哮喘小鼠Th17/Treg T细胞及其细胞因子白介素IL-17、IL-23、IL-10、转化生长因子-β1(TGF-β1)表达影响,探讨其可能作用机制。方法:选择8周龄健康雌性BALB/C小鼠,随机分为3组:对照组、支气管哮喘模型组、红景天组,每组10只;支气管哮喘模型采用卵清蛋白致敏方法构建,小鼠支气管灌洗液细胞(BALF)计数,ELISA法测定肺组织中IL-17、IL-23、IL-10、TGF-β1水平,流式细胞术测定外周血CD_4^+IL-17^+与FOXp_3^+CD_4^+CD_(25)^+占CD_4^+的比例,HE染色观察肺组织病理形态学变化。结果:与对照组比较,模型组BALF细胞总数、嗜酸性粒细胞、中性粒细胞、淋巴细胞数及肺匀浆IL-17、IL-23、CD_4^+IL-17^+占CD_4^+比例明显升高(P<0.05),肺匀浆IL-10、TGF-β1及FOXp_3^+CD_4^+CD_(25)^+占CD_4^+比例明显降低(P<0.05),肺组织炎症反应增加;与模型组比较,红景天组BALF细胞总数、嗜酸性粒细胞、中性粒细胞、淋巴细胞数及IL-17、IL-23、CD_4^+IL-17^+占CD_4^+比例明显降低(P<0.05),IL-10、TGF-β1及FOXp_3^+CD_4^+CD_(25)^+占CD_4^+比例明显升高(P<0.05),肺组织炎症反应减轻。结论:红景天可有效抑制哮喘小鼠气道炎症反应,其作用可能与调控哮喘小鼠Th17/Treg T细胞及其细胞因子IL-17、IL-23、IL-10、TGF-β1表达有关。
Objective： This aim was to investigate the effect of RhodiolaRosea on Th17/Treg cells and cytokines of IL-17,IL-23,IL-10 and transforming growth factor-β1（ TGF-β1） in bronchial asthma mice. Methods： Eight healthy female BALB/C mice were randomly divided into three groups： control group,bronchial asthma model group and Rhodiola group（ n = 10）. The bronchial asthma model was established by ovalbumin sensitization method. The levels of IL-17,IL-23,IL-10 and TGF-β1 in lung tissue were determined by ELISA and the levels of CD4^＋IL-17^＋ and FOXp3^＋CD4^＋CD25^＋ in peripheral blood were measured by flow cytometry. The pathological changes of lung tissue were observed by HE staining. Results： The number of BALF cells,the number of eosinophils,the level of IL-17,IL-23 andproportion of CD4~＋IL-17~＋ in the model group were significantly higher than those in the control group（ P〈0. 05）,while IL-10,TGF-β1 and FOXp3^＋CD4^＋CD25 in the modelgroupwere significantly decreased（ P〈0. 05）,the inflammatory reaction of lung tissue increased. Compared with the model group,the total number of BALF cells,eosinophils and IL-17,IL23,CD4^＋IL-17^＋ were significantly decreased（ P〈0. 05）,IL-10,TGF-β1 and FOXp3^＋CD4^＋CD25^＋reduced. Conclusion： The results show that Rhodiola can effectively inhibit the airway inflammation in asthmatic mice,and its effect may be related to the regulation of Th17/Treg T cells and the expression of IL-17,IL-23,IL-10 and TGF-β1 in asthmatic mice.
出处
《中华中医药学刊》
CAS
北大核心
2017年第6期1560-1563,I0033,共5页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省自然科学基金一般项目(LY17H290004)
