摘要
目的建立脑得生固体分散体胶囊中三七皂苷类成分(三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1)的含量测定方法。方法采用高效液相法,Hyspersil ODS-2色谱柱(5μm,250×4.6mm),柱温25℃,流动相乙腈-水系统梯度洗脱,流速0.9m L·min-1,检测波长203nm。结果三七皂苷R1在0.464~3.480μg(r=0.9999)范围,人参皂苷Rg1在1.968~14.760μg(r=0.9999)范围,人参皂苷Rb1在1.848~13.860μg(r=0.9999)范围均呈良好的线性关系;平均回收率分别为97.60%,99.22%,100.78%;RSD分别为1.86%,2.44%,1.84%。结论该方法简便、准确、重复性好,可用于该制剂的质量控制。
OBJECTIVE To simultaneous determine the contents of notoginsenoside R1 , ginsenoside Rg1 , gin- senoside RbI in Naodesheng solid dispersion capsules by HPLC. METHODS An external standard method by HPLC with Hyspersil ODS-2 C ls column （5 μm ,250mm × 4. 6mm） was used with the column temperature was 25 ℃. The detection wavelength was 203nm. The flow rate was set at 0. 9mL · min^-1 and gradient elution with acetonitrile and H20. RESULTS Linearities of notoginsenoside R1 ,ginsenoside Rg1 and ginsenoside Rb1 were good in the ran- ges of 0. 464 - 3.480 μg （ r = 0. 9999 ）, 1. 968 - 14. 760 μg （ r = 0. 9999 ） and 1. 848 - 13. 860μg （ r = 0. 9999 ）, re- spectively. The average recoveries of notoginsenoside R1, ginsenoside Rg1 and ginsenoside RbI were 97.60%, 99.22% ,100.78% and RSD were 1.86% ,2. 44% ,1.84% ,respectively. CONCLUSION The method is simple, accurate and reproducible, and can be used for quality control of Naodesheng solid dispersion capsules.
出处
《海峡药学》
2017年第5期43-46,共4页
Strait Pharmaceutical Journal
基金
国家自然科学基金面上项目(81274059)
