摘要
目的:探讨神经营养因子受体p75NTR在乳腺癌耐药细胞中的表达及其与多药耐药的相关性。方法:Western blot检测多种乳腺癌细胞系中p75NTR蛋白表达;基因重组构建p75NTR的正义及反义载体并检测转染p75NTR及p75NTR-si RNA后乳腺癌耐药细胞系MDA-MB-231/ADR中p75NTR蛋白表达;CCK-8法检测MDA-MB-231/ADR对各种化疗药物的敏感性及转染p75NTR及p75NTR-si RNA后对耐药的影响。结果:p75NTR在乳腺癌耐药细胞系中的表达高于亲本细胞;p75NTR过表达载体上调MDA-MB-231/ADR中p75NTR的表达;过表达p75NTR可增强MDA-MB-231/ADR对ADM、GEM和OXA的耐药性。结论:p75NTR在乳腺癌耐药细胞系中的表达高于其亲本细胞,过表达p75NTR能够降低多药耐药细胞对化疗药物的敏感性而促进其多药耐药性。
Objective To investigate the expression of neurotrophins receptor 75NTR in human breast cancer resistant cell line and its correlation with mnltidrug resistance. Methods Western blot was used to detect the expression level of p75NTR protein in various breast cancer cell lines and muhidrug resistant cell lines. The over-expression vector and siRNA vector of p75NTR were constructed by gene recombination method. Western blot was used to detect the expression levels of p75NTR protein in transfected p75NTR and p75NTR - siRNA breast cancer resistant cell line MDA-MB-231/ADR; the sensitivity of MDA-MB-231 and MDA-MB-231/ADR to different chemotherapeutic anticancer drug （FIX, ADM, GEM, DDP, OXA） and the multi-drug resistance effects in over-expression and knock-down p75NTR MDA-MB-231/ADR were detected by CCK-8 assay. Results Western blot result showed that the expressions of p75NTR protein in the mnhidrug-resistant breast cancer cell lines MDA- MB-231/ADR and MCF-7/5-FU were higher than that of other breast cancer cell lines. Over-expression of p75NTR can up-regulate the expression of p75NTR protein in MDA-MB-231/ADR; the CCK-8 assay indicated that overexpression of p75NTR can effectively enhance the resistance of MDA-MB-231/ADR cells to ADM, GEM and OXA. Conclusion The expression of p75NTR in breast cancer resistant cell line is higher than that of its parental cell line; over-expression of p75NTR can reduce the sensitivity to chemotherapeutic drugs and promote its multidrug resistance.
出处
《实用医学杂志》
CAS
北大核心
2017年第9期1389-1393,共5页
The Journal of Practical Medicine
基金
广东省医学科研基金资助项目(编号:B2016070)
广州医科大学科研资助项目(编号:2015C42)
