摘要
建立超高效液相色谱法(UPLC-PDA)同时测定藏药翼首草环烯醚萜和酚酸类10个化学成分的含量。采用Acquity UPLC^R BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱,流动相0.2%磷酸水(A)-乙腈(B),梯度洗脱,检测波长237,325 nm,流速0.4 mL·min^(-1),柱温30℃,进样量1μL。研究结果表明UPLC-PDA法定量分析翼首草10个化学成分在考察的范围内线性关系良好(r≥0.999 7);加样回收率为96.30%~103.0%(n=9),RSD为0.72%~2.9%(n=9)。所建立UPLC方法简便、准确、重复性好,可同时测定藏药翼首草中10个化学成分的含量。
This study is to develop an UPLC-PDA method for determination of 10 major components in Pterocephalus. The UPLC-PDA assay was performed on a Waters Acquity UPLCR BEH C18（2.1 mm×100 mm,1.7 μm）, and the column temperature was at 30℃. The mobile phase consists of water containing 0.2% phosphoric acid （A） and acetonitrile （B） in gradient elution at a flow rate of 0.4 mL·min^-1. The detection wave length was set at 237 and 325 nm, and the injection volume was 1 μL in the UPLC system. The linear range of 10 detected compounds were good （r≥0.999 7）, and the overall recoveries ranged from 96.30% to 103.0%, with the RSD ranging from 0.72% to 2.9%. The method was simple, accurate and reproducible, which can be used for the simultaneous determination of the content of ten major components in P. hookeri.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第7期1234-1237,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81274193)
四川省教育厅属高校科研创新团队建设计划项目(11TD004)
关键词
藏药
翼首草
UPLC-PDA
环烯醚萜
酚酸
含量测定
Tibetan medicine
Pterocephalus hookeri
UPLC-PDA
iridoid glycoside
phenolic acid
content determination
