摘要
目的观察磷酸肌酸钠(SP)对阿霉素(ADM)所致大鼠心肌损伤的保护作用,并探讨其可能的作用机制。方法应用随机数字表法将30只雄性SD大鼠分为空白对照组(n=10)、阿霉素组(ADR组,n=10)和磷酸肌酸钠组(SP组,n=10)。对照组腹腔注射生理盐水每日一次,ADR组隔日一次腹腔注射阿霉素建立阿霉素心肌损伤模型,SP组隔日一次腹腔注射磷酸肌酸钠(200 mg/kg),30 min后注射阿霉素。实验结束后应用羟胺法测定各组大鼠超氧化物歧化酶(SOD),应用硫代巴比妥法测定各组大鼠丙二醛(MDA),应用ELISA法测定各组心肌酶谱[乳酸脱氢酶(LDH)、心肌天冬氨酸转氨酶(AST)、磷酸肌酸激酶(CK)、肌酸激酶同功酶(CK-MB)]水平。结果 ADR组和SP组大鼠血清SOD水平分别为(47.11±9.64)μg/L、(80.9±17.98)μg/L,显著低于空白对照组的(89.12±18.23)μg/L,而MDA水平分别为(7.12±1.46)μg/L、(2.3±0.47)μg/L,显著高于空白对照组的(1.66±0.34)μg/L。SP组大鼠血清SOD水平高于ADR组,MDA水平低于ADR组,差异均有统计学意义(P<0.05)。ADR组和SP组大鼠血清心肌酶谱(LDH、AST、CK、CK-MB)水平分别为(1 307.79±267.50)U/L、(476.19±97.40)U/L、(961.29±196.63)U/L、(1 353.33±276.82)U/L和(713.79±146.02)U/L、(164.32±33.61)U/L、(387.09±79.18)U/L、(565.29±115.63)U/L,均明显高于空白对照组的(691.02±141.35)U/L、(130.68±26.73)U/L、(327.69±67.03)U/L、(487.08±99.63)U/L,而SP组大鼠的上述血清心肌酶谱水平均高于ADR组,差异均有统计学意义(P<0.05)。结论磷酸肌酸钠干预可以减轻大鼠阿霉素心肌损伤,其保护作用的实现,可能是由于清除了氧自由基,增强了SOD的活性,减轻脂质过氧化反应,并修复了心肌细胞损伤。
Objective To investigate the effect and possible mechanisms of sodium phosphocreatine（SP） to protect against adriamycin-induced cardiotoxicity injury in rats. Methods Thirty cases of male SD rats were randomly divided into blank control group（n=10, intraperitoneal injection of saline once a day）, adriamycin group（n=10, intraperitoneal injection of doxorubicin every other day to establish adriamycin myocardial injury model） and SP group（n=10, intraperitoneal injection of SP every other day 200 mg/kg 30 min after the injection of doxorubicin）. At the end of the experiment, the levels of superoxide dismutase（SOD） in each group were measured by hydroxylamine method. Malondialdehyde（MDA） was determined by thiobarbital method. The myocardial enzymes such as lactate dehydrogenase（LDH）,asparate aminotransferase（AST）, creatine kinase（CK）, creatine kinase-MB isoenzyme（CK-MB） levels were measured.Results The levels of serum SOD in ADR group and SP group were（47.11±9.64） μg/L,（80.9±17.98） μg/L, significantly lower than（89.12 ± 18.23） μg/L in control group（P〈0.05）. The levels of serum MDA in ADR group and SP group were（7.12±1.46） μg/L,（2.3±0.47） μg/L, significantly higher than（1.66±0.34） μg/L in control group（P〈0.05）. The level of serum SOD in SP group was higher than that in ADR group, and the level of MDA in SP group was significantly lower than that in ADR group（P〈0.05）. The levels of serum myocardial enzymes（LDH, AST, CK, CK-MB） were（1307.79±267.50）,（476.19±97.40）,（961.29±196.63） and（1 353.33±276.82） in ADR group and（713.79±146.02）,（164.32±33.61）,（387.09±79.18）,（565.29±115.63） in SP group, which were significantly higher than（691.02±141.35）,（130.68±26.73）,（327.69±67.03）,（487.08±99.63） in the control group（P〈0.05）. The levels of serum myocardial enzymes（LDH,AST, CK, CK-MB） in SP group were significantly higher than those in ADR group（P〈0.05）. Conclusion SP has protective effect on adriamycin-induced cardiotoxicity injury, maybe through scavenging oxygen free radicals, enhancing the SOD activity, reducing lipid peroxidation and repairing myocardial cell damage.
出处
《海南医学》
CAS
2017年第7期1033-1035,共3页
Hainan Medical Journal
关键词
SD大鼠
磷酸肌酸钠
阿霉素
心肌损伤
氧自由基
SD rats
Sodium phosphocreatine
Adriamycin
Myocardial injury
Oxygen free radicals
