摘要
为了找到适合芽胞漆酶固定化的载体和方法,对12种载体共19种芽胞漆酶固定化方案进行了对比研究,并通过参考固定化芽胞漆酶的酶活力回收率、重复利用率、成本等因素,选定以DEAE-纤维素为载体通过离子结合法固定为最佳方案。结果表明,包埋法固定化芽胞漆酶存在严重的扩散限制作用,部分包埋法制备的微球还会伴有溶解、膨胀的现象。而多孔材料常因孔径过小难以固定芽胞。芽胞以碳二亚胺为交联剂与载体进行共价结合,具有良好的固定化性能,可作为备选方案。
The comparative studies for immobilization of spore laccase were carried out using 19 different methods and 12 carriers. The optimal carrier and method were determined by the activity yield, reusability, and cost of the immobilized spore laccase. The ionic bonding method using DEAE-cellulose as the carrier was selected as the best method. The strong diffusion limitations were observed when the spore laccase was immobilized by embedding method. The microspheres might dissolve and swell i f semi-embedding method was used. The small pore size made it difficult for porous materials to immobilized spore laccase. The excellent immobilization properties were achieved when the spores were covalently bound to the carriers using carbodiimide as the crosslinker. It was thus recommended as an alternative method.
作者
周稳
管政兵
蔡宇杰
赵宏
廖祥儒
ZHOU Wen GUAN Zhengbing CAI Yujie ZHAO Hong LIAO Xiangrn(School of Biotechnology,Jiangnan University ,Wuxi 214122,China Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2017年第4期364-370,共7页
Journal of Food Science and Biotechnology
基金
国家自然科学基金项目(31101331)
江南大学工业生物技术教育部重点实验室开放课题(KF201304)
江苏高校优势学科建设工程资助项目
111引智计划(111-2-06)
江苏省现代发酵工业协同创新中心项目
关键词
芽胞漆酶
包埋法
吸附法
离子结合
共价结合
spore laccase, embedding, adsorption, ionic bonding, covalent bonding
