摘要
目的分析并探讨胶体金免疫层析法(GICA)检测登革热病毒NS1抗原和(或)IgG/IgM抗体的效果。方法选择我院2014年1月~2015年6月收治的1200例门诊疑似登革热病毒感染者作为研究对象,应用随机分组法将患者分为两组各600例,分别接受聚合酶链反应(PCR)荧光探针法和酶联免疫吸附测定法(ELISA),再将上述两类样本分别随机分为3份,每份200例,A组用GICA检测NSI抗原,B组用GICA检测IgG/IgM抗体,C组共同检测NS1抗原和IgG/IgM抗体。另D组选取60名健康人群使用GICA联合检测NS1抗原和IgG/IgM抗体。结果ELISA组结果:A组结果与ELISA结果相比,阳性、阴性、总体符合率分别为82.88%、90.00%、83.00%,结果有统计学差异(P<0.05);B组结果与ELISA结果相比,阳性、阴性、总体符合率分别为88.20%、71.79%、85.00%,结果有统计学差异(P<0.05);C组结果与ELISA结果相比,阳性、阴性、总体符合率分别为94.87%、47.73%、84.50%,结果无统计学差异(P>0.05);PCR组结果:A组结果与PCR结果相比,阳性、阴性、总体符合率分别为87.68%、83.87%、86.50%,结果有统计学差异;B组结果与PCR结果相比,阳性、阴性、总体符合率分别为91.45%、85.42%、90.00%,结果有统计学差异(P<0.05);C组结果与PCR结果相比,阳性、阴性、总体符合率分别为95.43%、60.00%、91.00%,结果无统计学差异(P>0.05);D组结果均为阴性,特异性为100%。结论 GICA对NIS抗原和IgG/IgM抗体的联合检测结果与ELISA和PCR检测结果较为接近,且特异性比较好,可用于登革热病毒感染的早期筛查和辅助诊断。
Objective To analyze and explore the effect of application of colloidal gold immunochromatography assay （GICA） in the detection of Dengue virus NS1 antigen and/or IgG/IgM antibody.Methods Altogether 1200 cases of suspected Dengue virus infection patients who were treated in our hospital from January 2014 to June 2015 were involved as the object of this research and divided into two groups （PCR group and ELISA group） by using the random grouping method, with 600 casesin each group.Two groups were treated with polymerase chain reaction（PCR） fluorescence probe and enzyme-linked immunosorbent assay （ELISA）,respectively.The two types of samples aforementioned were randomly divided into 3 sub-groups,with 200 samples in each sub-groups：GICA was used in group A to detect NSI antigen;GICA was used in group B to detect IgG/IgM antibody;it was also used in group C for detection of NS1 antigen and IgG/IgM antibody.Additionally,60 healthy volunteers were selected as Group D,whose NS1 antigen and IgG/IgM antibody were detected by using GICA.Results ELISA group resuhs：eompared with the results of ELISA group, the positive,negative and overall coincidence rate of group A were 82.88%,90.00% and 83.00%,with statistically significant difference（P〈0.05）;compared with the results of ELISA group,the positive,negative and overall coincidence rate of group B were 88.20%,71.79% and 85.00%,with statistically significant difference （P〈0.05）;compared with the results of ELISA Group,the positive,negative and overall coincidence rate of group A were 94.87%,47.73% and 84.50%,without statistically significant difference （P〉0.05）;PCR group results：compared with the results of PCR group,the positive, negative and overall coincidence rate of group A were 87.68%, 83.87% and 86.50%,with statistically significant differene （P〈 0.05）;compared with the results of PCR group,the positive,negative and overall coincidence rate of group B were 91.45%,85.42% and 90%,with statistically significant difference （P〈0.05）;compared with the results of PCR Group,the positive, negative and overall coincidence rate of Group B were 95.43%,60.00% and 91.00%,without statistically significant difference （P〉0.05）.The result of Group D was negative and its specificity was 100%.Conclusion The result achieved by application of GICA is close to that of ELISA and PCR in detection of NIS and IgG/IgM with good specificity.tcan be used for early screening and diagnosis of dengue virus infections.
出处
《中国当代医药》
2017年第12期156-158,163,共4页
China Modern Medicine
基金
广东省广州市黄埔区科技计划项目(201544-17)
关键词
胶体金免疫层析法
酶联免疫吸附测定法
聚合酶链反应
登革热病毒
Colloidal gold immunochromatographic assay
Enzyme-linked immunosorbent assay
Polymerase chain reaction
Dengue virus
