摘要
目的 证实大鼠坐骨神经上ATP受体P2y的分布。 方法 梨状肌下缘以远 5mm处切取长 5mm的坐骨神经 ,同时切取主动脉 3mm作阳性对照 ;组织总mRNA提取 ,RT PCRcDNA第一链合成 ,反应所得产物于 2 %琼脂糖凝胶电泳 ,1μg/ml溴化乙啶染色 (EB) ,观察 ,照相。 结果 在大鼠坐骨神经上有 3种ATP受体亚型表达 (P2y2、P2y4和P2y6mRNA表达 )。 结论 大鼠坐骨神经上有 3种ATP受体亚型分布 ,细胞外ATP可能通过此三种受体或其中之一完成轴突诱向作用 。
Objective To demonstrate the presentation of the extracellula ATP receptors in peripheral nerve in rats Methods Reverse transcriptase polymerase chain reaction was performed in sciatic nerve of rat Results There were three P2y receptor mRNA expressions in the in the sciatic nerve of the rat,they were P2y2 and P2y4 as well as P2y6 Conclusions Extracellular ATP,as a neurotransmitter,activates the P2y purinoceptors located on the axon regeneration after peripheral nerve injury and decided the direction of the regeneration axon The P2y receptors are related to the regeneration of the peripheral nerve
出处
《中华显微外科杂志》
CSCD
北大核心
2002年第3期201-203,共3页
Chinese Journal of Microsurgery
