摘要
目的通过调查江苏地区汉族人群人类白细胞抗原-E(HLA-E)等位基因多态性分布情况,为探讨HLA-E基因多态性与疾病关联奠定基础。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)方法,调查217例江苏地区汉族献血人群HLA-E的表达。结果检测到HLA-E的2个等位基因,分别是HLA-E*01:01和HLA-E*01:03,分布频率为41.47%和58.53%;基因型HLA-E*01:01/01:01、01:01/01:03和01:03/01:03的频率分别为14.74%、53.46%和31.80%,分布符合Hardy-weinberg平衡定律。结论江苏地区汉族人群HLA-E高度保守,仅有2种基因型。
Objective To investigate the genetic polymorphisms of HLA-E locus in Han population of Jiangsu region. Methods The genomic DNA extracted from 217 unrelated healthy blood donors were amplified by using HLA-E specific PCR primers. Results Two alleles including HLA-E＊ 01： 01 and HLA-E＊ 01： 03 were identified, the frequencies were 41.47% and 58.53%, respectively. The frequencies of the three HLA-E genotypes （HLA-E＊01： 01/01： 01, 01： 01 /01： 03 and 01： 03/01： 03） were 14.74%, 53.46% and 31.80%, respectively, and they were in accordancewith Hardy-Weinberg Equilibrium. Conclusion The polymorphism of HLA-E in the Han population of Jiangsu province was highly conserved, suggesting a role of HLA-E in disease association and clinical transplantation.
出处
《临床输血与检验》
CAS
2017年第2期173-175,共3页
Journal of Clinical Transfusion and Laboratory Medicine
基金
江苏省"六大人才高峰"项目(No.WSN-2014-002)资助
